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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Rabbit Anti-Human IgG Fc - AP 抗体
- 抗体英文名:
Rabbit Anti-Human IgG Fc - AP
- 浓度:
详询
- 宿主:
Rabbit
- 保质期:
6-12个月
- 抗原来源:
详询
- 目录编号:
orb1786494
- 级别:
科研
- 库存:
99
- 供应商:
Biorbyt
- 标记物:
AP
- 保存条件:
详询
- 形态:
详询
- 免疫原:
详询
- 规格:
500 ul/100 ul
| 规格: | 500 ul | 产品价格: | ¥5489.0 |
|---|---|---|---|
| 规格: | 100 ul | 产品价格: | ¥2101.0 |
Rabbit Anti-Human IgG Fc - AP
产品名:Rabbit Anti-Human IgG Fc - AP
货号:orb1786494
产品中文名:Rabbit Anti-Human IgG Fc - AP 抗体
产品详情链接:https://www.biorbyt.com/rabbit-anti-human-igg-fc-ap-orb1786494.html
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文献和实验which employs goat anti-Rabbit HRP.12. Wash filter 3 times 7 minutes with 0.5% BSA in TBST.13. Perform Alkaline phosphatase reaction using NBT and BCIP substrates. I find that CREB usually comes up within several minutes of adding the AP substrates
% nonfat dry milk works well for anti-PCREB, but in my hands milk is not as good as BSA from USB (it seems to mask the epitope). Remove block solution and add primary antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000
. 3- add the AP conjugated secondary antibody (50 to 100 µl) and incubate for 45 min. The secondary antibody should be absorbed against human serum; if not add 1% human serum before use. SBA Goat anti mouse AP or Goat anti Rabbit AP can be used 1:200
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