Detection of human PTEN by western blot. Samples: Whole cell lysate (50 µg) from HeLa, OVCAR-3, SR, MCF-7, and NCI-H460 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PTEN recombinant monoclonal antibody (orb1784572) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of mouse and rat PTEN by western blot. Samples: Whole cell lysate (50 µg) from 70Z/3, NIH 3T3, Ba/F3, EL4, and C6 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PTEN recombinant monoclonal antibody (orb1784572) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.