Detection of human NEDD-9/CASL/HEF1 by western blot. Samples: Whole cell lysate (50 µg) from OVCAR-8, U-251MG, A-549, and UACC-257 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-NEDD-9/CASL/HEF1 recombinant monoclonal antibody (orb1733239) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of mouse NEDD-9/CASL/HEF1 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3 and C2C12 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-NEDD-9/CASL/HEF1 recombinant monoclonal antibody (orb1733239) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human NEDD-9/CASL/HEF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction