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文献和实验Intraspinal Transplantation of Mouse and Human Neural Precursor Cells
.A., Bucci, M., Caverzasi, E., Gaetano, L., Mandelli, M.L., Ryan, T., Perry, R., Farrell, J., Jeremy, R.J., Ulman, M., Huhn, S.L., Barkovich, A.J., and Rowitch, D.H. 2012. Neural stem cell engraftment and myelination in the human brain. Sci. Transl. Med
monoclonal anti-HA 16B12 (MMS101R, BAbCo, used at 1:200 to 1:1000 dilution) mouse monoclonal anti-HA 12CA5 (12CA5, Boehringer, used at 1:200 dilution) rabbit polyclonal anti-HA (RS1010C, BAbCo, used at 1:200 dilution) Cy3-conjugated affinity
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
. A cheap alternative is to exploit the relative lower levels of each IgG isotype in serum and on follicular B cells to stain with isotype-specific, conjugated, anti-mouse secondary antibodies. IgG isotypes account each for 20-25% ot total serum
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