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文献和实验Isolation of RNA from Difficult Tissues
. Therefore, care should be taken during the initial preparation of the homogenate to ensure intact total RNA. Brain and Plant Tissues: Protein and Lipid-rich Tissues Isolating total RNA from rat or mouse brain can be very rewarding
Reproducible purification of DNA and RNA in 96-well format. Genomic DNA and total RNA were purified from mouse tissues using the AllPrep DNA/RNA 96 Kit. A Purified DNA samples were analyzed on a 0.8% agarose gel. B
分离培养大鼠或小鼠小脑神经元 Dissociated Cultures of Cerebellar Neurons
also use Sprague-Dawley and Wistar rats yield of cells is approx 1.5 X 107 cells per P8 rat original protocols call for fetal bovine serum; however, calf serum was used and seems satisfactory insulin is used for survival, rather than IGF-1 of ref
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