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- 保存条件:
Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
- 英文名:
Human CD152 Protein, His Tag
- 库存:
999
- 供应商:
卡梅德生物科技(天津)有限公司
- 规格:
10ug/50ug
| 规格: | 10ug | 产品价格: | ¥640.0 |
|---|---|---|---|
| 规格: | 50ug | 产品价格: | ¥1536.0 |
产品编号:KMP2425
产品名称:Human CD152 Protein, His Tag
中文名称: 人CTLA-4/CD152蛋白
中文全称:人细胞毒T淋巴细胞相关抗原/CD152蛋白
产品描述:The Human CD152 Protein(KMP2425) is produced in HEK293 Cells and the target gene encoding Lys36-Asp161 is expressed with a 6His tag at the C-terminus.
产品别称:细胞毒T淋巴细胞相关抗原, Cytotoxic T-lymphocyte protein 4, CD152
分子名称:CD152
预测分子量:14.33 kDa
物种:Human
宿主:HEK293 Cells
规格:50ug, 100ug, 200ug
纯化方式:Affinity purification
纯度:>95% as determined by SDS-PAGE
内毒素水平:<1.0 EU/ug determined by the LAL method
缓冲体系:PBS, pH7.4
Uniport:E9L3G3#function
产品背景:Cytotoxic Tlymphocyte 4(CTLA-4,CD152), is a type I transmembrane T cell inhibitory molecule that is a member of the Ig superfamily. Human or mouse CTLA4 cDNA encodes 223 amino acids(aa) including a 35 aa signal sequence, a 126 aa extracellular domain(ECD) with one Ig-like V-type domain, a 21 aa transmembrane(TM) sequence, and a 41 aa cytoplasmic sequence.It is widely expressed with highest levels in lymphoid tissues. CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin(Ig) gene superfamily. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T Cells and may play an important role in their functions. Tcell activation through the Tcell receptor and CD28 leads to increased expression of CTLA4.
保存条件:Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
运输条件:In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
说明:This product is for research use only.
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高表达CD25的调节性T细胞,造成部分调节性T细胞的丢失。调节性T细胞在细胞表面及胞浆内表达CTLA-4(CD152),它是细胞免疫的负反馈调节因子;表达TNFRSF-18,也称作GITR,属于TNF超家族。活化的非调节性T细胞表达会增加表达CD25、GITR和CD152,说明这些分子也不能用于定义调节性T细胞,且现在发现不是所有的调节性T细胞都会表达以上这些分子。如能发现一种唯一性的调节性T细胞标记物有助于解决以上所有的问题。 CD127表达识别人调节性T细胞 目前为止,识别
x 106/ml; 3.4 加入1 000 U/ml 的重组人IFN-g培养; 3.5 24h 后加入50ng/ml 的CD3 单克隆抗体和300 U/ml 的重组人IL-2,刺激CIK 细胞的生长和增殖; 注:此时也可同时加入100 U/ml的重组人IL-1a。 3.6 每3天半量换液或扩瓶一次,并补加重组人IL-2 300 U/ml; 3.7 在培养的第7d,收获CIK细胞,此时数量应达到1x 109个以上。 3.8 CIK细胞质控: 3.8.1 台盼蓝染色检测
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