Detection of mouse PDGFR beta by western blot. Samples: Whole cell lysate (10 µg) from A20, NIH 3T3, C6, C2C12, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (orb1570943) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of mouse PDGFR beta (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (orb1570943) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Detection of mouse PDGFR beta by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction
Detection of mouse PDGFR beta by immunocytochemistry. Sample: FFPE section of mouse CT26 cells. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (orb1570943). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse PDGFR beta by immunocytochemistry. Sample: FFPE section of mouse C2C12 cells. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (orb1570943). Secondary: HRP-conjugated goat anti-rabbit IgG.