A synthetic peptide corresponding to a sequence at the C-terminus of human WDR1 (552-579aa MDMMVYVWTLSDPETRVKIQDAHRLHHV), different from the related mouse and rat sequences by one amino acid.
内容
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
纯化方式
protein G/A purified
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
WD repeat-containing protein 1 is a protein that in humans is encoded by the WDR1 gene. It is mapped to 4p16.1. This gene encodes a protein containing 9 WD repeats. WD repeats are approximately 30- to 40-amino acid domains containing several conserved residues, mostly including a trp-asp at the C-terminal end. WD domains are involved in protein-protein interactions. The encoded protein may help induce the disassembly of actin filaments. Two transcript variants encoding different isoforms have been found for this gene.
研究类别
1. "Entrez Gene: WDR1 WD repeat domain 1".2. Adler HJ, Winnicki RS, Gong TW, Lomax MI (May 1999). "A gene upregulated in the acoustically damaged chick basilar papilla encodes a novel WD40 repeat protein". Genomics 56 (1): 59–69.3. Fujibuchi T, Abe Y, Takeuchi T, et al. (2005). "AIP1/WDR1 supports mitotic cell rounding". Biochem. Biophys. Res. Commun. 327 (1): 268–75.
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.
*稀释度需要用户自己调试,此处数据仅供参考。
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[list_product_images]Figure 1. Western blot analysis of anti- WDR1 antibody (M04814-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: U87 whole cell lysate,
Lane 2: A549 whole cell lysate,
Lane 3: mouse lung tissue lysate,
Lane 4: mouse liver tissue lysate.
Use mouse anti- WDR1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for WDR1 at approximately 66KD. The expected band size for WDR1 is at 66KD.|Figure 2. IHC analysis of WDR1 using anti-WDR1 antibody (M04814-1).
WDR1 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.|Figure 3. IHC analysis of WDR1 using anti-WDR1 antibody (M04814-1).
WDR1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1022) as the chromogen.[/list_product_images]