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广州威佳科技有限公司
150ul
货号 | MA00165-1 |
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产品名称 | Anti-IRF3 Antibody (Clone#OTI4D4) |
基因名 | IRF3 |
抗体来源 | Mouse |
克隆 | OTI4D4 |
抗体亚型 | Mouse IgG2a |
分子量 | 47KD |
免疫原 | Full length human recombinant protein of human IRF3 (NP_001562) produced in HEK293T cell. |
内容 | PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
纯化方式 | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
浓度 | 500 ug/ml |
产品形态 | Liquid |
保存条件 | Stable for 12 months from date of receipt. Store at -20°C as received. |
背景资料 | IRF3(interferon regulatory factor 3)?is a member of the interferon regulatory transcription factor(IRF) family. The IRF3 gene is mapped on 19q13.33. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with?CREBBP. IRF3 plays an important role in the?innate immune system's response to?viral infection. Aggregated?MAVS?have been found to activate IRF3 dimerization. Although?IRF3?increased transcriptional activity from an ISRE-containing promoter, expression of?IRF3?as a Gal4 fusion protein did not activate expression of a chloramphenicol acetyltransferase(CAT) reporter gene containing repeats of the Gal4-binding sites.?Translocation of?IRF3?was accompanied by an increase in serine and threonine phosphorylation.?The transcriptional activators CREBBP and EP300 coimmunoprecipitated with?IRF3?only subsequent to viral infection, and the authors stated that these are also subunits of DRAF1. |
研究类别 | 1. Bellingham, J., Gregory-Evans, K., Gregory-Evans, C. Y. Mapping of human interferon regulatory factor 3 (IRF3) to chromosome 19q13.3-13.4 by an intragenic polymorphic marker. Ann. Hum. Genet. 62: 231-234, 1998.2. Doyle, S. E., Vaidya, S. A., O'Connell, R., Dadgostar, H., Dempsey, P. W., Wu, T.-T., Rao, G., Sun, R., Haberland, M. E., Modlin, R. L., Cheng, G.IRF3 mediates a TLR3/TLR4-specific antiviral gene program. Immunity 17: 251-263, 2002.3. Stetson, D. B., Medzhitov, R. Recognition of cytosolic DNA activates an IRF3-dependent innate immune response. Immunity 24: 93-103, 2006. |
Uniprot ID | IRF3: Q14653 |
为了提供优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
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Western blot (WB): | 1:1000~2000 |
Immunohistochemistry in paraffin section (IHC): | 1:50 |
Immunofluorescence (IF): | 1:100 |
Flow cytometry (FCM): | 1:100 |
*稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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