Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
500 ug/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]
研究类别
1. Jacobs MD, Harrison SC (1998). "Structure of an IkappaBalpha/NF-kappaB complex". Cell 95 (6): 749–58.2. Neish, A. S.., Gewirtz, A. T., Zeng, H., Young, A. N., Hobert, M. E., Karmali, V., Rao, A. S., Madara, J. L. Prokaryotic regulation of epithelial responses by inhibition of I-kappa-B-alpha ubiquitination. Science 289: 1560-1563, 2000.3. Verma IM, Stevenson JK, Schwarz EM, Van Antwerp D, Miyamoto S (1995). "Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation". Genes Dev.9 (22): 2723–35.
[list_product_images]Figure 1. Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT) and NFKBIA-Knockout 293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-NFKBIA monoclonal antibody MA01139, (1:500. Then the blotted membrane was stripped and reprobed with anti-b-actin antibody ([MA01263]) as a loading control.|Figure 2. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NFKBIA monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).|Figure 3. Immunofluorescent staining of HeLa cells using anti-NFKBIA mouse monoclonal antibody.|Figure 4. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NFKBIA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NFKBIA. [/list_product_images]