E.coli-derived human SLC25A29 recombinant protein (Position: H20-E242).
内容
500 ug/ml antibody with PBS,0.02% NaN3, 1 mg BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Solute carrier family 25, member 29 is a protein that in humans is encoded by the SLC25A29 gene. This gene encodes a nuclear-encoded mitochondrial protein that is a member of the large family of solute carrier family 25 (SLC25) mitochondrial transporters. The members of this superfamily are involved in numerous metabolic pathways and cell functions. This gene product was previously reported to be a mitochondrial carnitine-acylcarnitine-like (CACL) translocase (PMID:128829710) or an ornithine transporter (designated ORNT3, PMID:19287344), however, a recent study characterized the main role of this protein as a mitochondrial transporter of basic amino acids, with a preference for arginine and lysine (PMID:24652292). Alternatively spliced transcript variants have been found for this gene.
研究类别
1. Camacho, J. A., Rioseco-Camacho, N. The human and mouse SLC25A29 mitochondrial transporters rescue the deficient ornithine metabolism in fibroblasts of patients with the hyperornithinemia-hyperammonemia-homocitrullinuria (HHH) syndrome. Pediat. Res. 66: 35-41, 2009.2. Sekoguchi, E., Sato, N., Yasui, A., Fukada, S., Nimura, Y., Aburatani, H., Ikeda, K., Matsuura, A. A novel mitochondrial carnitine-acylcarnitine translocase induced by partial hepatectomy and fasting. J. Biol. Chem. 278: 38796-38802, 2003.
[list_product_images]Figure 1. Western blot analysis of anti- SLC25A29 antibody (A11796-1). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human MOLT-4 whole cell lysates,
Lane 3: human Hela whole cell lysates.
Use rabbit anti- SLC25A29 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for SLC25A29 at approximately 35KD. The expected band size for SLC25A29 is at 32KD.[/list_product_images]