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150ul
货号 | M00833-1 |
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产品名称 | Anti-YY1 Antibody (monoclonal, 6H3E1) |
基因名 | YY1 |
抗体来源 | Mouse |
克隆 | Monoclonal(Clone:6H3E1) |
抗体亚型 | Mouse IgG2b |
分子量 | 65KD |
免疫原 | A synthetic peptide corresponding to a sequence in the middle region of human YY1 (206-241aa EQKQVQIKTLEGEFSVTMWSSDEKKDIDHETVVEEQ), identical to the related mouse sequence. |
内容 | 500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol. |
纯化方式 | protein G purified. |
浓度 | 500 ug/ml |
产品形态 | Liquid |
保存条件 | 12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
背景资料 | YY1 (Yin Yang 1) is a transcriptional repressor protein in humans that is encoded by the YY1 gene. YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1. |
研究类别 | 1. Shi Y, Seto E, Chang LS, Shenk T (Oct 1991). "Transcriptional repression by YY1, a human GLI-Krüppel-related protein, and relief of repression by adenovirus E1A protein". Cell 67 (2): 377–88.2. Thiaville MM, Kim J (2011). "Oncogenic potential of yin yang 1 mediated through control of imprinted genes". Critical Reviews in Oncogenesis 16 (3-4): 199–209.3. Zhu W, Lossie AC, Camper SA, Gumucio DL (Apr 1994). "Chromosomal localization of the transcription factor YY1 in the mouse and human". Mammalian Genome 5 (4): 234–6. |
Uniprot ID | YY1: P25490 |
推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P). |
为了提供优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
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Western blot(WB): | 1:500-2000 |
Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
Flow cytometry (FCM): | 1-3μg/1x106 cells |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
*稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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