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150ul
货号 | A00512-2 |
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产品名称 | Anti-GJB2 Antibody |
基因名 | GJB2 |
抗体来源 | Rabbit |
克隆 | Polyclonal |
抗体亚型 | Rabbit IgG |
分子量 | 26KD |
免疫原 | A synthetic peptide corresponding to a sequence of human GJB2 (FYVMYDGFSMQRLVKCN). |
内容 | 500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol. |
纯化方式 | Immunogen affinity purified. |
浓度 | 500 ug/ml |
产品形态 | Liquid |
保存条件 | 12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
背景资料 | Connexin26(CX26), also known as GAP junction protein, beta2, GJB2. Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels. Proteins, called connexins, purified from fractions of enriched gap junctions from different tissues differ. The 3-prime untranslated region of the CX26 transcript contains a putative mRNA instability sequence. The deduced 226-amino acid protein has a calculated molecular mass of about 26 kD. CX26 shares 92.5% identity with rat Cx26. connexin 26(GJB2) is assigned to human chromosome 13q11-q12 .Connexin 26 regulates epidermal barrier and wound remodeling and promotes psoriasiform response. Connexin 26 gene(GJB2) mutation modulates the severity of hearing loss associated with the 1555A-G mitochondrial mutation. |
研究类别 | 1. Mignon, C.; Fromaget, C.; Mattei, M.-G.; Gros, D.; Yamasaki, H.; Mesnil, M. : Assignment of connexin 26 (GJB2) and 46 (GJA3) genes to human chromosome 13q11-q12 and mouse chromosome 14D1-E1 by in situ hybridization. Cytogenet. Cell Genet. 72: 185-186, 1996. 2. Djalilian, A. R.; McGaughey, D.; Patel, S.; Seo, E. Y.; Yang, C.; Cheng, J.; Tomic, M.; Sinha, S.; Ishida-Yamamoto, A.; Segre, J. A. : Connexin 26 regulates epidermal barrier and wound remodeling and promotes psoriasiform response. J. Clin. Invest. 116: 1243-1253, 2006. 3. Abe, S.; Kelley, P. M.; Kimberling, W. J.; Usami, S. : Connexin 26 gene (GJB2) mutation modulates the severity of hearing loss associated with the 1555A-G mitochondrial mutation. Am. J. Med. Genet. 103: 334-338, 2001. |
Uniprot ID | GJB2: P29033 |
推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. |
为了提供优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
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Western blot(WB): | 1:500-2000 |
Flow cytometry (FCM): | 1-3μg/1x106 cells |
*稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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