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150ul
货号 | A11954-1 |
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产品名称 | Anti-NeuN Antibody |
基因名 | RBFOX3 |
抗体来源 | Rabbit |
克隆 | Polyclonal |
抗体亚型 | Rabbit IgG |
分子量 | 46-55KD |
免疫原 | E.coli-derived mouse NeuN/Rbfox3 recombinant protein (Position: H28-D304). |
内容 | 500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol. |
纯化方式 | Immunogen affinity purified. |
浓度 | 500 ug/ml |
产品形态 | Liquid |
保存条件 | 12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
背景资料 | RNA binding protein, fox-1 homolog (C. elegans) 3 (Rbfox3) is a protein that in humans is encoded by the RBFOX3 gene. This gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. |
研究类别 | 1. Dredge, B. K., Jensen, K. B. NeuN/Rbfox3 nuclear and cytoplasmic isoforms differentially regulate alternative splicing and nonsense-mediated decay of Rbfox2. PLoS One 6: e21585, 2011. Note: Electronic Article. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 6/22/2016.3. Kim, K. K., Adelstein, R. S., Kawamoto, S. Identification of neuronal nuclei (NeuN) as Fox-3, a new member of the Fox-1 gene family of splicing factors. J. Biol. Chem. 284: 31052-31061, 2009. |
Uniprot ID | RBFOX3: Q8BIF2 |
推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. |
为了提供优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
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Western blot (WB): | 1:500-2000 |
Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
Immunofluorescence (IF): | 1:50-400 |
ELISA: | 1:100-1000 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
*稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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