A synthesized peptide derived from human LGR5/GPR49
内容
500 ug/ml;Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
纯化方式
Affinity-chromatography
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Leucine?rich repeat G?protein?coupled receptor 5 (Lgr5), also called GPR49, is a 907 amino acid (aa), approximately 97 kDa (calculated), seven?transmembrane glycoprotein receptor in the Lgr family of cell surface receptors. The subfamily of Lgrs comprising Lgr4, Lgr5, and Lgr6 are G?protein?independent mediators of the potentiating effect of R?Spondins on Wnt signaling (2). Lgr5 binds and forms complexes with R?Spondins, Frizzled Wnt receptors and LRP Wnt co?receptors. The region of the human Lgr5 long extracellular domain used as an immunogen shares 90% amino acid sequence identity with mouse and rat Lgr5, respectively. Lgr5 is found on embryonic and adult epithelial stem cells (3). Lgr5+ stem cells can produce all epithelial cell types of the intestinal crypts (4). Abnormal LGR5 expression and regulation in stem cells might give rise to cancers such as intestinal, hepatocellular, pancreatic and ovarian carcinomas (5,6). This antibody has been referred to as "RD42" in Peng et al. (1).