A synthetic peptide corresponding to a sequence in the middle region of mouse Sca1/Ly6A/E (48-75aa YPDGVCVTQEAAVIVDSQTRKVKNNLCL).
内容
500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Stem cell antigen-1 (Sca-1) is a mouse glycosyl phosphatidylinositol-anchored protein and a cell surface marker found on hematopoietic stem cells (HSCs). It is encoded by the strain-specific Ly-6 E/A allelic gene. Sca-1 has been discovered in several non hematopoietic tissues and can be used to enrich progenitor cell populations other than HSC. It is suggested that Sca-1 could be involved in regulating both B and T cell activation.
研究类别
1. Codias, E.K. et al. (1990) J. Immunol. 144:2197.2. Malek, T.R. et al. (1986) J. Exp. Med. 164:709 .
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
[list_product_images]Figure 1. Western blot analysis of Sca1/Ly6A/E using anti- Sca1/Ly6A/E antibody (A30403). Lane 1: HEPA1-6 whole cell lysates. anti- Sca1/Ly6A/E antigen affinity purified polyclonal antibody (Catalog # A30403)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for Sca1/Ly6A/E at approximately 17KD. The expected band size for Sca1/Ly6A/E is at 14KD.|Figure 2. IHC analysis using anti- Sca1/Ly6A/E antibody (A30403). detected in paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.[/list_product_images]