E.coli-derived human SENP1 recombinant protein (Position: N19-P619).
内容
500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Sentrin-specific protease 1 is a protein that in human is encoded by the SENP1 gene. This gene is mapped to 12q13.11. This gene encodes a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family. This protease regulates SUMO pathways by deconjugating sumoylated proteins. This protease also functions to process the precursor SUMO proteins into their mature form. Alternate splicing results in multiple transcript variants.
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
[list_product_images]Figure 1. Western blot analysis of SENP1 using anti-SENP1 antibody (A02156-3).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human CACO-2 whole cell lysates,Lane 2: human HEPG2 whole cell lysates,Lane 3: human HELA whole cell lysates,Lane 4: human Jurkat whole cell lysates,Lane 5: human U87 whole cell lysates,Lane 6: human Raji whole cell lysates,Lane 7: human HL-60 whole cell lysates.Use rabbit anti-SENP1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for SENP1 at approximately 73KD. The expected band size for SENP1 is at 73KD.[/list_product_images]