E. coli-derived mouse CD31 recombinant protein (Position: R58-Q273).
内容
500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
CD31 also known as Platelet endothelial cell adhesion molecule (PECAM-1), is a protein that in human is encoded by the PECAM1 gene. Encoded protein is a member of the immunoglobulin superfamily and this gene is mapped to 17q23.3. CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is demonstrated that CD31 expression on human PBSCs may positively affect both neutrophil and platelet engraftment. Meanwhile, CD31 is involved in leukocyte migration and angiogenesis, which are key components of venous thrombus resolution.
研究类别
1. Donmez A, Tombuloglu M, Gulbahar O, Arik B, Cagirgan S, Vural F, Gokmen N."CD31 expression on peripheral blood stem cells predicts both early neutrophil and platelet engraftments."2013 Oct;49(2):307-12. 2. Gumina RJ, Kirschbaum NE, Rao PN, vanTuinen P, Newman PJ (June 1996). "The human PECAM1 gene maps to 17q23". Genomics 34 (2): 229–32.3. Kellermair J, Redwan B, Alias S, Jabkowski J, Panzenboeck A, Kellermair L, Winter MP, Weltermann A, Lang IM."Platelet endothelial cell adhesion molecule 1 deficiency misguides venous thrombus resolution."2013 Nov 7; 122(19):3376-84. 4. Newman PJ, Berndt MC, Gorski J, White GC, Lyman S, Paddock C, Muller WA (March 1990). "PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily". Science 247 (4947): 1219–22.5. Xie Y, Muller WA (October 1996). "Fluorescence in situ hybridization mapping of the mouse platelet endothelial cell adhesion molecule-1 (PECAM1) to mouse chromosome 6, region F3-G1". Genomics 37 (2): 226–8.
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
[list_product_images]Figure 1. Western blot analysis of CD31 using anti-CD31 antibody (A01513-1). Lane 1: mouse ovary tissue lysates,Lane 2: mouse stomach tissue lysates,Lane 3: mouse kidney tissue lysates. anti-CD31 antigen affinity purified polyclonal antibody (Catalog # A01513-1)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for CD31 at approximately 120-130KD. The expected band size for CD31 is at 82KD.[/list_product_images]