E.coli-derived human COX2/Cyclooxygenase 2/PTGS2 recombinant protein (Position: A18-L604).
内容
500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
At -20℃ for one year. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for a longer time. Avoid repeated freezing and thawing.
背景资料
Prostaglandin-endoperoxide synthase 2,also known as cyclooxygenase-2 or COX-2,is an enzyme that in humans is encoded by the PTGS2 gene. It is mapped to 1q31.1. Prostaglandin-endoperoxide synthase (PTGS),also known as cyclooxygenase,is the key enzyme in prostaglandin biosynthesis,and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2,which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events,suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis.
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
[list_product_images]Figure 1. Western blot analysis of anti- PTGS2 antibody (A00084-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: mouse RAW264.7(-LPS) whole cell lysates,
Lane 3: mouse RAW264.7(+LPS) whole cell lysates.
Use rabbit anti- PTGS2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for PTGS2 at approximately 75KD. The expected band size for PTGS2 is at 69KD.|Figure 2. Flow cytometry analysis of CACO-2 cell(1x106) DyLight488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).|Figure 3. Flow cytometry analysis of HEPA1-6 cell(1x106) DyLight488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).[/list_product_images]