- ￥1960
- BOSTER
- PB9359-100ul
- 中国
- 2025年07月02日
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- 技术资料
- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | PB9359 |
|---|---|
| 产品名称 | Anti-vimentin Antibody |
| 基因名 | VIM |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 56KD |
| 免疫原 | A synthetic peptide corresponding to a sequence at the C-terminus of human Vimentin (435-466aa DTHSKRTLLIKTVETRDGQVINETSQHHDDLE), identical to the related mouse and rat sequences. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | VIM(vimentin) is also known as HEL113 or CTRCT30. This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract. |
| 研究类别 | 1. Besarani D, et al. Role of anti-vimentin antibodies in renal transplantation. Transplantation, 2014 Jul 15.2. Kinloch AJ, et al. Vimentin is a dominant target of in situ humoral immunity in human lupus tubulointerstitial nephritis. Arthritis Rheumatol, 2014 Dec.3. Shirahata A, et al. Serum vimentin methylation as a potential marker for colorectal cancer. Anticancer Res, 2014 Aug. |
| Uniprot ID | VIM: P08670 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:1000-5000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti-Vimentin antibody (PB9359). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human U-87MG whole cell lysates,
Lane 3: human U20S whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: human Hela whole cell lysates,
Lane 6: human THP-1 whole cell lysates,
Lane 7: human A549 whole cell lysates,
Lane 8: human MCF-7 whole cell lysates,
Lane 9: rat ovary tissue lysates,
Lane 10: rat lung tissue lysates,
Lane 11: mouse lung tissue lysates,
Lane 12: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Vimentin antigen affinity purified polyclonal antibody (PB9359) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Vimentin at approximately 56 kDa. The expected band size for Vimentin is at 54 kDa.|Figure 2. IHC analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of mouse cardiac muscle tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 3. IHC analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of rat cardiac muscle tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 4. IHC analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 5. IHC analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of human placenta tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 6. IHC analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 7. IHC analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 8. IHC analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of human invasive urothelial carcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 9. IHC analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 10. IHC analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 11. IF analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of mouse brain tissue. DyLight?488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).|Figure 12. IF analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of rat brain tissue. DyLight?488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).[/list_product_images]
Lane 1: human placenta tissue lysates,
Lane 2: human U-87MG whole cell lysates,
Lane 3: human U20S whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: human Hela whole cell lysates,
Lane 6: human THP-1 whole cell lysates,
Lane 7: human A549 whole cell lysates,
Lane 8: human MCF-7 whole cell lysates,
Lane 9: rat ovary tissue lysates,
Lane 10: rat lung tissue lysates,
Lane 11: mouse lung tissue lysates,
Lane 12: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Vimentin antigen affinity purified polyclonal antibody (PB9359) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Vimentin at approximately 56 kDa. The expected band size for Vimentin is at 54 kDa.|Figure 2. IHC analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of mouse cardiac muscle tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 3. IHC analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of rat cardiac muscle tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 4. IHC analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 5. IHC analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of human placenta tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 6. IHC analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 7. IHC analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 8. IHC analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of human invasive urothelial carcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 9. IHC analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 10. IHC analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 11. IF analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of mouse brain tissue. DyLight?488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).|Figure 12. IF analysis of Vimentin using anti-Vimentin antibody (PB9359).
Vimentin was detected in a paraffin-embedded section of rat brain tissue. DyLight?488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).[/list_product_images]
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Anti-Vimentin Antibody(原货号PB0378)(PB9359-100ul)
￥1960
