- ￥1960
- BOSTER
- A11908-2-100ul
- 中国
- 2025年07月12日
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- 库存：
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A11908-2 |
|---|---|
| 产品名称 | Anti-POLR1B Antibody |
| 基因名 | POLR1B |
| 抗体来源 | Rabbit |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 128KD |
| 免疫原 | E.coli-derived human POLR1B recombinant protein (Position: H18-V1135). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | DNA-directed RNA polymerase I subunit RPA2 is an enzyme that in humans is encoded by the POLR1B gene. Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2. |
| 研究类别 | 1. Sanchez, E., Laplace-Builhe, B., Mau-Them, F. T., Richard, E., Goldenberg, A., Toler, T. L., Guignard, T., Gatinois, V., Vincent, M., Blanchet, C., Boland, A., Bihoreau, M. T., and 16 others. POLR1B and neural crest cell anomalies in Treacher Collins syndrome type 4. Genet. Med. 22: 547-556, 2020. 2. Seither, P., Grummt, I. Molecular cloning of RPA2, the gene encoding the second largest subunit of mouse RNA polymerase I. Genomics 37: 135-139, 1996.3. Stumpf, A. M. Personal Communication. Baltimore, Md. 07/06/2020. |
| Uniprot ID | POLR1B: Q9H9Y6 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry(Intracellular): | 1-3 μg/1x106 cells |
| Direct ELISA: | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of anti-POLR1B antibody (A11908-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-POLR1B antigen affinity purified polyclonal antibody (A11908-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for POLR1B at approximately 128 kDa. The expected band size for POLR1B is at 128 kDa.|Figure 2. IHC analysis of POLR1B using anti-POLR1B antibody (A11908-2).
POLR1B was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 3. IHC analysis of POLR1B using anti-POLR1B antibody (A11908-2).
POLR1B was detected in a paraffin-embedded section of human lymphoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 4. IF analysis of POLR1B using anti-POLR1B antibody (A11908-2).
POLR1B was detected in an immunocytochemical section of PC-3 cells. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) (Catalog # BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).|Figure 5. Flow Cytometry analysis of A431 cells using anti-POLR1B antibody (A11908-2).
Overlay histogram showing A431 cells stained with A11908-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-POLR1B Antibody (A11908-2, 1 μg/1x106 cells). DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
Lane 1: human K562 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-POLR1B antigen affinity purified polyclonal antibody (A11908-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for POLR1B at approximately 128 kDa. The expected band size for POLR1B is at 128 kDa.|Figure 2. IHC analysis of POLR1B using anti-POLR1B antibody (A11908-2).
POLR1B was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 3. IHC analysis of POLR1B using anti-POLR1B antibody (A11908-2).
POLR1B was detected in a paraffin-embedded section of human lymphoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 4. IF analysis of POLR1B using anti-POLR1B antibody (A11908-2).
POLR1B was detected in an immunocytochemical section of PC-3 cells. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) (Catalog # BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).|Figure 5. Flow Cytometry analysis of A431 cells using anti-POLR1B antibody (A11908-2).
Overlay histogram showing A431 cells stained with A11908-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-POLR1B Antibody (A11908-2, 1 μg/1x106 cells). DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
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Anti-POLR1B Antibody(A11908-2-100ul)
￥1960
