- ￥1960
- BOSTER
- A10793-100ul
- 中国
- 2025年07月11日
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大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A10793 |
|---|---|
| 产品名称 | Anti-Plasmolipin/PLLP Antibody |
| 基因名 | PLLP |
| 抗体来源 | Rabbit |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 26KD |
| 免疫原 | E.coli-derived human Plasmolipin/PLLP recombinant protein (Position: M1-A142). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | PLLP (Plasmolipin, also known as PMLP; TM4SF11), located on 16q13, is a Protein Coding gene. The gene produces a 19987 Da protein composed of 182 amino acids. Plasmolipin (PLLP) is a novel protein identified in human corneal epithelium and has been demonstrated to have a key role in epithelial cell differentiation in other tissues. Plasmolipin is a hydrophobic plasma membrane proteolipid present in both the kidney and brain. The mouse plasmolipin gene has the common organization of the 4-transmembrane (4TM) gene group, with 4 exons and a large first intron. |
| 研究类别 | 1. Fischer, I., Sapirstein, V. S. Molecular cloning of plasmolipin: characterization of a novel proteolipid restricted to brain and kidney. J. Biol. Chem. 269: 24912-24919, 1994. 2. Hamacher, M., Pippirs, U., Kohler, A., Muller, H. W., Bosse, F. Plasmolipin: genomic structure, chromosomal localization, protein expression pattern, and putative association with Bardet-Biedl syndrome. Mammalian Genome 12: 933-937, 2001. |
| Uniprot ID | PLLP: Q9Y342 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Flow Cytometry(Intracellular): | 1-3 μg/1x106 cells |
| Direct ELISA: | 1:100-1000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti-Plasmolipin/PLLP antibody (A10793). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Plasmolipin/PLLP antigen affinity purified polyclonal antibody (A10793) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Plasmolipin/PLLP at approximately 26 kDa. The expected band size for Plasmolipin/PLLP is at 18,20 kDa.|Figure 2. Flow Cytometry analysis of HepG2 cells using anti-Plasmolipin/PLLP antibody (A10793).
Overlay histogram showing HepG2 cells stained with A10793 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Plasmolipin/PLLP Antibody (A10793, 1 μg/1x106 cells). DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
Lane 1: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Plasmolipin/PLLP antigen affinity purified polyclonal antibody (A10793) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Plasmolipin/PLLP at approximately 26 kDa. The expected band size for Plasmolipin/PLLP is at 18,20 kDa.|Figure 2. Flow Cytometry analysis of HepG2 cells using anti-Plasmolipin/PLLP antibody (A10793).
Overlay histogram showing HepG2 cells stained with A10793 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Plasmolipin/PLLP Antibody (A10793, 1 μg/1x106 cells). DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
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Anti-Plasmolipin/PLLP Antibody(A10793-100ul)
￥1960
