- ￥1960
- BOSTER
- A08649-100ul
- 中国
- 2025年07月11日
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- 技术资料
- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A08649 |
|---|---|
| 产品名称 | Anti-LYPLA3/PLA2G15 Antibody |
| 基因名 | PLA2G15 |
| 抗体来源 | Rabbit |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 42-57KD |
| 免疫原 | E.coli-derived human LYPLA3/PLA2G15 recombinant protein (Position: D92-Q372). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | LYPLA3, also known as Group XV phospholipase A2, is an enzyme that in humans is encoded by the PLA2G15 gene. Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene hydrolyzes lysophosphatidylcholine to glycerophosphorylcholine and a free fatty acid. This enzyme is present in the plasma and thought to be associated with high-density lipoprotein. A later paper contradicts the function of this gene. It demonstrates that this gene encodes a lysosomal enzyme instead of a lysophospholipase and has both calcium-independent phospholipase A2 and transacylase activities. |
| 研究类别 | 1. Abe, A., Poucher, H. K., Hiraoka, M., Shayman, J. A. Induction of lysosomal phospholipase A2 through the retinoid X receptor in THP-1 cells. J. Lipid Res. 45: 667-673, 2004. 2. Hiraoka, M., Abe, A., Shayman, J. A. Cloning and characterization of a lysosomal phospholipase A2, 1-O-acylceramide synthase. J. Biol. Chem. 277: 10090-10099, 2002. 3. Taniyama, Y., Shibata, S., Kita, S., Horikoshi, K., Fuse, H., Shirafuji, H., Sumino, Y., Fujino, M. Cloning and expression of a novel lysophospholipase which structurally resembles lecithin cholesterol acyltransferase. Biochem. Biophys. Res. Commun. 257: 50-56, 1999. |
| Uniprot ID | PLA2G15: Q8NCC3 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Direct ELISA: | 1:100-1000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti-LYPLA3/PLA2G15 antibody (A08649). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human SiHa whole cell lysates,
Lane 3: human U251 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LYPLA3/PLA2G15 antigen affinity purified polyclonal antibody (A08649) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for LYPLA3/PLA2G15 at approximately 42-57 kDa. The expected band size for LYPLA3/PLA2G15 is at 65-70 kDa.|Figure 2. IF analysis of LYPLA3/PLA2G15 using anti-LYPLA3/PLA2G15 antibody (A08649) anti-Tubulin Alpha antibody (M03989-3).
LYPLA3/PLA2G15 was detected in an immunocytochemical section of U2OS cells. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) (Catalog # BA1032) and Dylight488-conjugated Anti-mouse IgG Secondary Antibody (Green) (Catalog # BA1126) were used as secondary antibody.[/list_product_images]
Lane 1: human placenta tissue lysates,
Lane 2: human SiHa whole cell lysates,
Lane 3: human U251 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LYPLA3/PLA2G15 antigen affinity purified polyclonal antibody (A08649) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for LYPLA3/PLA2G15 at approximately 42-57 kDa. The expected band size for LYPLA3/PLA2G15 is at 65-70 kDa.|Figure 2. IF analysis of LYPLA3/PLA2G15 using anti-LYPLA3/PLA2G15 antibody (A08649) anti-Tubulin Alpha antibody (M03989-3).
LYPLA3/PLA2G15 was detected in an immunocytochemical section of U2OS cells. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) (Catalog # BA1032) and Dylight488-conjugated Anti-mouse IgG Secondary Antibody (Green) (Catalog # BA1126) were used as secondary antibody.[/list_product_images]
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Anti-LYPLA3/PLA2G15 Antibody(A08649-100ul)
￥1960
