- ￥1960
- BOSTER
- A16917-1-100ul
- 中国
- 2025年07月10日
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- 技术资料
- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A16917-1 |
|---|---|
| 产品名称 | Anti-PPM1N Antibody |
| 基因名 | PPM1N |
| 抗体来源 | Rabbit |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 45KD |
| 免疫原 | E.coli-derived human PPM1N recombinant protein (Position: R36-A430). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Predicted to enable metal ion binding activity and protein serine/threonine phosphatase activity. Predicted to be involved in negative regulation of I-kappaB kinase/NF-kappaB signaling and positive regulation of canonical Wnt signaling pathway. Predicted to be active in cytosol and nucleus. |
| 研究类别 | 1. Zhou, H. , Xu, M. , Huang, Q. , Gates, A. T. , Zhang, X. D. , & Castle, J. C. , et al. (2008). Genome-scale rnai screen for host factors required for hiv replication. Cell host & microbe, 4(5), 495-504.2. Shi, P. , Guo, Y. , Su, Y. , Zhu, M. , & Huang, J. . (2020). Sumoylation of ddx39a alters binding and export of antiviral transcripts to control innate immunity. The Journal of Immunology, 205(1), ji2000053.3. Fasci, D. , Ingen, H. V. , Scheltema, R. A. , & Heck, A. J. R. . (2018). Histone interaction landscapes visualized by crosslinking mass spectrometry in intact cell nuclei. Molecular & Cellular Proteomics Mcp, 17(10), mcp.RA118.000924. |
| Uniprot ID | PPM1N: Q8N819 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry(Intracellular): | 1-3 μg/1x106 cells |
| Direct ELISA: | 1:100-1000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti-PPM1N antibody (A16917-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PPM1N antigen affinity purified polyclonal antibody (A16917-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PPM1N at approximately 45 kDa. The expected band size for PPM1N is at 46 kDa.|Figure 2. IF analysis of PPM1N using anti-PPM1N antibody (A16917-1).
PPM1N was detected in an immunocytochemical section of A549 cells. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue). |Figure 3. Flow Cytometry analysis of MCF-7 cells using anti-PPM1N antibody (A16917-1).
Overlay histogram showing MCF-7 cells stained with A16917-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPM1N Antibody (A16917-1, 1 μg/1x106 cells). DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
Lane 1: human RT4 whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PPM1N antigen affinity purified polyclonal antibody (A16917-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PPM1N at approximately 45 kDa. The expected band size for PPM1N is at 46 kDa.|Figure 2. IF analysis of PPM1N using anti-PPM1N antibody (A16917-1).
PPM1N was detected in an immunocytochemical section of A549 cells. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue). |Figure 3. Flow Cytometry analysis of MCF-7 cells using anti-PPM1N antibody (A16917-1).
Overlay histogram showing MCF-7 cells stained with A16917-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPM1N Antibody (A16917-1, 1 μg/1x106 cells). DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
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Anti-PPM1N Antibody(A16917-1-100ul)
￥1960
