- ￥1960
- BOSTER
- A10969-2-100ul
- 中国
- 2025年07月11日
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- 库存：
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A10969-2 |
|---|---|
| 产品名称 | Anti-PRDM8 Antibody |
| 基因名 | PRDM8 |
| 抗体来源 | Rabbit |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 70KD |
| 免疫原 | E.coli-derived human PRDM8 recombinant protein (Position: D14-D405). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | This gene encodes a protein that belongs to a conserved family of histone methyltransferases that predominantly act as negative regulators of transcription. The encoded protein contains an N-terminal Su(var)3-9, Enhancer-of-zeste, and Trithorax (SET) domain and a double zinc-finger domain. Knockout of this gene in mouse results in mistargeting by neurons of the dorsal telencephalon, abnormal itch-like behavior, and impaired differentiation of rod bipolar cells. In humans, the protein has been shown to interact with the phosphatase laforin and the ubiquitin ligase malin, which regulate glycogen construction in the cytoplasm. Alternative splicing results in multiple transcript variants. |
| 研究类别 | 1. Eom, G. H., Kim, K., Kim, S.-M., Kee, H. J., Kim, J.-Y., Jin, H. M., Kim, J.-R., Kim, J. H., Choe, N., Kim, K.-B., Lee, J., Kook, H., Kim, N., Seo, S.-B. Histone methyltransferase PRDM8 regulates mouse testis steroidogenesis. Biochem. Biophys. Res. Commun. 388: 131-136, 2009.2. Hartz, P. A. Personal Communication. Baltimore, Md. 11/11/2015.3. Komai, T., Iwanari, H., Mochizuki, Y., Hamakubo, T., Shinkai, Y. Expression of the mouse PR domain protein Prdm8 in the developing central nervous system. Gene Expr. Patterns 9: 503-514, 2009. |
| Uniprot ID | PRDM8: Q9NQV8 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry(Intracellular): | 1-3 μg/1x106 cells |
| Direct ELISA: | 1:100-1000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti-PRDM8 antibody (A10969-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PRDM8 antigen affinity purified polyclonal antibody (A10969-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PRDM8 at approximately 70 kDa. The expected band size for PRDM8 is at 72 kDa.|Figure 2. IF analysis of PRDM8 using anti-PRDM8 antibody (A10969-2) and anti-Tubulin alpha antibody (M03989-3).
PRDM8 was detected in an immunocytochemical section of A431 cells. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) and Dylight488-conjugated Anti-mouse IgG Secondary Antibody (Green) (Catalog # BA1126) were used as secondary antibody.|Figure 3. Flow Cytometry analysis of A431 cells using anti-PRDM8 antibody (A10969-2).
Overlay histogram showing A431 cells stained with A10969-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRDM8 Antibody (A10969-2, 1 μg/1x106 cells). DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
Lane 1: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PRDM8 antigen affinity purified polyclonal antibody (A10969-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PRDM8 at approximately 70 kDa. The expected band size for PRDM8 is at 72 kDa.|Figure 2. IF analysis of PRDM8 using anti-PRDM8 antibody (A10969-2) and anti-Tubulin alpha antibody (M03989-3).
PRDM8 was detected in an immunocytochemical section of A431 cells. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) and Dylight488-conjugated Anti-mouse IgG Secondary Antibody (Green) (Catalog # BA1126) were used as secondary antibody.|Figure 3. Flow Cytometry analysis of A431 cells using anti-PRDM8 antibody (A10969-2).
Overlay histogram showing A431 cells stained with A10969-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRDM8 Antibody (A10969-2, 1 μg/1x106 cells). DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
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Anti-PRDM8 Antibody(A10969-2-100ul)
￥1960
