- ￥1960
- BOSTER
- A10327-1-100ul
- 中国
- 2025年07月10日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A10327-1 |
|---|---|
| 产品名称 | Anti-PYCR2 Antibody |
| 基因名 | PYCR2 |
| 抗体来源 | Rabbit |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 35KD |
| 免疫原 | E.coli-derived human PYCR2 recombinant protein (Position: Q10-D320). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Pyrroline-5-carboxylate reductase family, member 2 is a protein that in humans is encoded by the PYCR2 gene. This gene belongs to the pyrroline-5-carboxylate reductase family. The encoded mitochondrial protein catalyzes the conversion of pyrroline-5-carboxylate to proline, which is the last step in proline biosynthesis. Alternatively spliced transcript variants have been described for this gene. |
| 研究类别 | 1. De Ingeniis, J., Ratnikov, B., Richardson, A. D., Scott, D. A., Aza-Blanc, P., De, S. K., Kazanov, M., Pellecchia, M., Ronai, Z., Osterman, A. L., Smith, J. W. Functional specialization in proline biosynthesis of melanoma. PLoS One 7: e45190, 2012. Note: Electronic Article.2. Escande-Beillard, N., Loh, A., Saleem, S. N., Kanata, K., Hashimoto, Y., Altunoglu, U., Metoska, A., Grandjean, J., Ng, F. M., Pomp, O., Baburajendran, N., Wong, J., Hill, J., Beillard, E., Cozzone, P., Zaki, M., Kayserili, H., Hamada, H., Shiratori, H., Reversade, B. Loss of PYCR2 causes neurodegeneration by increasing cerebral glycine levels via SHMT2. Neuron 107: 82-94, 2020. 3. Nakayama, T., Al-Maawali, A., El-Quessny, M., Rajab, A., Khalil, S., Stoler, J. M., Tan, W.-H., Nasir, R., Schmitz-Abe, K., Hill, R. S., Partlow, J. N., Al-Saffar, M., and 10 others. Mutations in PYCR2, encoding pyrroline-5-carboxylate reductase 2, cause microcephaly and hypomyelination. Am. J. Hum. Genet. 96: 709-719, 2015. |
| Uniprot ID | PYCR2: Q96C36 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry(Intracellular): | 1-3 μg/1x106 cells |
| Direct ELISA: | 1:100-1000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti-PYCR2 antibody (A10327-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human THP-1 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat thymus tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PYCR2 antigen affinity purified polyclonal antibody (A10327-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PYCR2 at approximately 35 kDa. The expected band size for PYCR2 is at 34,35 kDa.|Figure 2. IF analysis of PYCR2 using anti-PYCR2 antibody (A10327-1).
PYCR2 was detected in an immunocytochemical section of U2OS cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue). |Figure 3. Flow Cytometry analysis of Jurkat cells using anti-PYCR2 antibody (A10327-1).
Overlay histogram showing Jurkat cells stained with A10327-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PYCR2 Antibody (A10327-1, 1 μg/1x106 cells). DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human THP-1 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat thymus tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PYCR2 antigen affinity purified polyclonal antibody (A10327-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PYCR2 at approximately 35 kDa. The expected band size for PYCR2 is at 34,35 kDa.|Figure 2. IF analysis of PYCR2 using anti-PYCR2 antibody (A10327-1).
PYCR2 was detected in an immunocytochemical section of U2OS cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue). |Figure 3. Flow Cytometry analysis of Jurkat cells using anti-PYCR2 antibody (A10327-1).
Overlay histogram showing Jurkat cells stained with A10327-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PYCR2 Antibody (A10327-1, 1 μg/1x106 cells). DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
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Anti-PYCR2 Antibody(A10327-1-100ul)
￥1960
