- ￥1960
- BOSTER
- A12839-1-100ul
- 中国
- 2025年07月10日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 技术资料
- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A12839-1 |
|---|---|
| 产品名称 | Anti-POLR2D Antibody |
| 基因名 | POLR2D |
| 抗体来源 | Rabbit |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 18KD |
| 免疫原 | E.coli-derived human POLR2D recombinant protein (Position: D7-D124). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | DNA-directed RNA polymerase II subunit RPB4 is an enzyme that in humans is encoded by the POLR2D gene. This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene. |
| 研究类别 | 1. Khazak, V., Estojak, J., Cho, H., Majors, J., Sonoda, G., Testa, J. R., Golemis, E. A. Analysis of the interaction of the novel RNA polymerase II (pol II) subunit hsRPB4 with its partner hsRPB7 and with pol II. Molec. Cell. Biol. 18: 1935-1945, 1998. |
| Uniprot ID | POLR2D: O15514 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Direct ELISA: | 1:100-1000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
点击图片放大
[list_product_images]Figure 1. Western blot analysis of anti-POLR2D antibody (A12839-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-POLR2D antigen affinity purified polyclonal antibody (A12839-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for POLR2D at approximately 18 kDa. The expected band size for POLR2D is at 88 kDa.|Figure 2. IF analysis of POLR2D using anti-POLR2D antibody (A12839-1) and anti-Beta Tubulin antibody (M01857-3).
POLR2D was detected in an immunocytochemical section of U2OS cells. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) (Catalog # BA1032) and Dylight488-conjugated Anti-mouse IgG Secondary Antibody (Green) (Catalog # BA1126) were used as secondary antibody. [/list_product_images]
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-POLR2D antigen affinity purified polyclonal antibody (A12839-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for POLR2D at approximately 18 kDa. The expected band size for POLR2D is at 88 kDa.|Figure 2. IF analysis of POLR2D using anti-POLR2D antibody (A12839-1) and anti-Beta Tubulin antibody (M01857-3).
POLR2D was detected in an immunocytochemical section of U2OS cells. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) (Catalog # BA1032) and Dylight488-conjugated Anti-mouse IgG Secondary Antibody (Green) (Catalog # BA1126) were used as secondary antibody. [/list_product_images]
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
技术资料暂无技术资料 索取技术资料
Anti-POLR2D Antibody(A12839-1-100ul)
￥1960
