- ￥1960
- BOSTER
- A15083-1-100ul
- 中国
- 2025年07月14日
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- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A15083-1 |
|---|---|
| 产品名称 | Anti-PRRC1 Antibody |
| 基因名 | PRRC1 |
| 抗体来源 | Rabbit |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 47KD |
| 免疫原 | E.coli-derived human PRRC1 recombinant protein (Position: Y139-D411). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | PRRC1 (Proline-Rich Coiled-Coil 1) is a Protein Coding gene. The PRRC1 gene, located on 5q23.2, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, and frog. The PRCC1 gene has two distinct regions: a proline-rich region on the N-terminus, and the DUF84 region on the C-terminus. PRCC1 is the commonly identified protein name of CAD38605. It encodes for 445 amino acids for a predicted total of 6 exons. The predicted molecular weight is 46.7 kDa, and the isoelectric point is 5.46. Orthologs have been determined in most eukaryotes, the most highly conserved being found in most mammalian species. PRRC1 is widely expressed in the thyroid, endometrium, and other tissues. Diseases associated with PRRC1 include Dystonia 23. |
| 研究类别 | 1. Kamakari, S. , Roussou, A. , Jefferson, A. , Ragoussis, I. , & Anagnou, N. P. . (2005). Structural analysis and expression profile of a novel gene on chromosome 5q23 encoding a golgi-associated protein with six splice variants, and involved within the 5q deletion of a ph(-) cml patient. Leukemia Research, 29(1), 17-31.2. WW Domains Provide a Platform for the Assembly of Multiprotein Networks.[J]. Molecular & Cellular Biology, 2005.3. St-Denis, N. , Gupta, G. D. , Lin, Z. Y. , Gonzalez-Badillo, B. , Pelletier, L. , & Gingras, A. C. . (2015). Myotubularin-related proteins 3 and 4 interact with polo-like kinase 1 and centrosomal protein of 55 kda to ensure proper abscission. Molecular & Cellular Proteomics. |
| Uniprot ID | PRRC1: Q96M27 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry(Intracellular): | 1-3 μg/1x106 cells |
| Direct ELISA: | 1:100-1000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of anti-PRRC1 antibody (A15083-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PRRC1 antigen affinity purified polyclonal antibody (A15083-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PRRC1 at approximately 47 kDa. The expected band size for PRRC1 is at 47 kDa.|Figure 2. IF analysis of PRRC1 using anti-PRRC1 antibody (A15083-1).
PRRC1 was detected in an immunocytochemical section of Hela cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).|Figure 3. Flow Cytometry analysis of U251 cells using anti-PRRC1 antibody (A15083-1).
Overlay histogram showing U251 cells stained with A15083-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRRC1 Antibody (A15083-1, 1 μg/1x106 cells). DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PRRC1 antigen affinity purified polyclonal antibody (A15083-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PRRC1 at approximately 47 kDa. The expected band size for PRRC1 is at 47 kDa.|Figure 2. IF analysis of PRRC1 using anti-PRRC1 antibody (A15083-1).
PRRC1 was detected in an immunocytochemical section of Hela cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).|Figure 3. Flow Cytometry analysis of U251 cells using anti-PRRC1 antibody (A15083-1).
Overlay histogram showing U251 cells stained with A15083-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRRC1 Antibody (A15083-1, 1 μg/1x106 cells). DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
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Anti-PRRC1 Antibody(A15083-1-100ul)
￥1960
