- ￥1960
- BOSTER
- A08890-100ul
- 中国
- 2025年07月14日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A08890 |
|---|---|
| 产品名称 | Anti-SUMF2 Antibody |
| 基因名 | SUMF2 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 36KD |
| 免疫原 | E.coli-derived human SUMF2 recombinant protein (Position: Q26-L301). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Sulfatase-modifying factor 2 is an enzyme that in humans is encoded by the SUMF2 gene. The catalytic sites of sulfatases are only active if they contain a unique amino acid, C-alpha-formylglycine (FGly). The FGly residue is posttranslationally generated from a cysteine by enzymes with FGly-generating activity. The gene described in this record is a member of the sulfatase-modifying factor family and encodes a protein with a DUF323 domain that localizes to the lumen of the endoplasmic reticulum. This protein has low levels of FGly-generating activity but can heterodimerize with another family member - a protein with high levels of FGly-generating activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
| 研究类别 | 1. Cosma, M. P., Pepe, S., Annunziata, I., Newbold, R. F., Grompe, M., Parenti, G., Ballabio, A. The multiple sulfatase deficiency gene encodes an essential and limiting factor for the activity of sulfatases. Cell 113: 445-456, 2003.2. Dierks, T., Schmidt, B., Borissenko, L. V., Peng, J., Preusser, A., Mariappan, M., von Figura, K. Multiple sulfatase deficiency is caused by mutations in the gene encoding the human C-alpha-formylglycine generating enzyme. Cell 113: 435-444, 2003. 3. Gross, M. B. Personal Communication. Baltimore, Md. 2/26/2015. |
| Uniprot ID | SUMF2: Q8NBJ7 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| ELISA: | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of anti- SUMF2 antibody (A08890). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates.
Use rabbit anti- SUMF2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for SUMF2 at approximately 36KD. The expected band size for SUMF2 is at 34KD.|Figure 2. IHC analysis using anti- SUMF2 antibody (A08890). detected in paraffin-embedded section of human breast cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 3. IHC analysis using anti- SUMF2 antibody (A08890). detected in paraffin-embedded section of human colon adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 4. IHC analysis using anti- SUMF2 antibody (A08890). detected in paraffin-embedded section of human ovarian tumor tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 5. IHC analysis using anti- SUMF2 antibody (A08890). detected in paraffin-embedded section of human placenta tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 6. IHC analysis using anti- SUMF2 antibody (A08890). detected in paraffin-embedded section of human squamous cell carcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 7. IF analysis of SUMF2 using anti-SUMF2 antibody (A00570-1).
SUMF2 was detected in a paraffin-embedded section of human colon cancer tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue). |Figure 8. IF analysis of SUMF2 using anti-SUMF2 antibody (A00570-1).
SUMF2 was detected in a paraffin-embedded section of human ovarian cancer tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue). |Figure 9. IF analysis of SUMF2 using anti-SUMF2 antibody (A00570-1).
SUMF2 was detected in a paraffin-embedded section of human placenta tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue). |Figure 10. Flow cytometry analysis of U-87 MG cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
Lane 1: human A431 whole cell lysates.
Use rabbit anti- SUMF2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for SUMF2 at approximately 36KD. The expected band size for SUMF2 is at 34KD.|Figure 2. IHC analysis using anti- SUMF2 antibody (A08890). detected in paraffin-embedded section of human breast cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 3. IHC analysis using anti- SUMF2 antibody (A08890). detected in paraffin-embedded section of human colon adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 4. IHC analysis using anti- SUMF2 antibody (A08890). detected in paraffin-embedded section of human ovarian tumor tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 5. IHC analysis using anti- SUMF2 antibody (A08890). detected in paraffin-embedded section of human placenta tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 6. IHC analysis using anti- SUMF2 antibody (A08890). detected in paraffin-embedded section of human squamous cell carcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 7. IF analysis of SUMF2 using anti-SUMF2 antibody (A00570-1).
SUMF2 was detected in a paraffin-embedded section of human colon cancer tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue). |Figure 8. IF analysis of SUMF2 using anti-SUMF2 antibody (A00570-1).
SUMF2 was detected in a paraffin-embedded section of human ovarian cancer tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue). |Figure 9. IF analysis of SUMF2 using anti-SUMF2 antibody (A00570-1).
SUMF2 was detected in a paraffin-embedded section of human placenta tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue). |Figure 10. Flow cytometry analysis of U-87 MG cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
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Anti-SUMF2 Antibody(A08890-100ul)
￥1960
