- ￥1960
- BOSTER
- A03624-1-100ul
- 中国
- 2025年07月15日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A03624-1 |
|---|---|
| 产品名称 | Anti-SA2/STAG2 Antibody |
| 基因名 | STAG2 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 141KD |
| 免疫原 | E.coli-derived human SA2/STAG2 recombinant protein (Position: E750-M1175). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Cohesin subunit SA-2 (SA2) is a protein that in humans is encoded by the STAG2 gene. The protein encoded by this gene is a subunit of the cohesin complex, which regulates the separation of sister chromatids during cell division. Targeted inactivation of this gene results in chromatid cohesion defects and aneuploidy, suggesting that genetic disruption of cohesin is a cause of aneuploidy in human cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| 研究类别 | 1. Aoi, H., Lei, M., Mizuguchi, T., Nishioka, N., Goto, T., Miyama, S., Suzuki, T., Iwama, K., Uchiyama, Y., Mitsuhashi, S., Itakura, A., Takeda, S., Matsumoto, N. Nonsense variants in STAG2 result in distinct sex-dependent phenotypes. J. Hum. Genet. 64: 487-492, 2019. Note: Retraction: J. Hum. Genet. 65: 811 only, 2020. 2. Balbas-Martinez, C., Sagrera, A., Carrillo-de-Santa-Pau, E., Earl, J., Marquez, M., Vazquez, M., Lapi, E., Castro-Giner, F., Beltran, S., Bayes, M., Carrato, A., Cigudosa, J. C., and 21 others. Recurrent inactivation of STAG2 in bladder cancer is not associated with aneuploidy. Nature Genet. 45: 1464-1469, 2013. 3. Carramolino, L., Lee, B. C., Zaballos. A., Peled, A., Barthelemy, I., Shav-Tal, Y., Prieto, I., Carmi, P., Gothelf, Y., Gonzalez de Buitrago, G., Aracil, M., Marquez, G., Barbero, J. L., Zipori, D. SA-1, a nuclear protein encoded by one member of a novel gene family: molecular cloning and detection in hemopoietic organs. Gene 195: 151-159, 1997. Note: Erratum: Gene 206: 283 only, 1998. |
| Uniprot ID | STAG2: Q8N3U4 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| ELISA: | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of anti- SA2/STAG2 antibody (A03624-1). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human MOLT-4 whole cell lysates,
Lane 2: human SiHa whole cell lysates,
Lane 3: human RT4 whole cell lysates.
Use rabbit anti- SA2/STAG2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for SA2/STAG2 at approximately 141KD. The expected band size for SA2/STAG2 is at 141KD.|Figure 2. IHC analysis using anti- SA2/STAG2 antibody (A03624-1). detected in paraffin-embedded section of mouse brain tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 3. IHC analysis using anti- SA2/STAG2 antibody (A03624-1). detected in paraffin-embedded section of rat brain tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 4. IHC analysis using anti- SA2/STAG2 antibody (A03624-1). detected in paraffin-embedded section of human colon adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 5. IHC analysis using anti- SA2/STAG2 antibody (A03624-1). detected in paraffin-embedded section of human lung squamous cell carcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 6. IHC analysis using anti- SA2/STAG2 antibody (A03624-1). detected in paraffin-embedded section of human ovarian cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 7. IHC analysis using anti- SA2/STAG2 antibody (A03624-1). detected in paraffin-embedded section of human penis squamous cell carcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 8. IHC analysis using anti- SA2/STAG2 antibody (A03624-1). detected in paraffin-embedded section of human placenta tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 9. IHC analysis using anti- SA2/STAG2 antibody (A03624-1). detected in paraffin-embedded section of human liver cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 10. IHC analysis using anti- SA2/STAG2 antibody (A03624-1). detected in paraffin-embedded section of human colon cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 11. IF analysis of SA2/STAG2 using anti-SA2/STAG2 antibody (A03624-1).
SA2/STAG2 was detected in a paraffin-embedded section of human colon cancer tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).|Figure 12. ICC analysis using anti- SA2/STAG2 antibody (A03624-1) and anti-Tubulin alpha antibody (M03989-3). were detected in immersion fixed SiHa cell line. Cells were stained using the Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) and Dylight488-conjugated Anti- mouse IgG Secondary Antibody (green)(Catalog#BA1126).|Figure 13. Flow cytometry analysis of 293T cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 14. Flow cytometry analysis of U937 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
Lane 1: human MOLT-4 whole cell lysates,
Lane 2: human SiHa whole cell lysates,
Lane 3: human RT4 whole cell lysates.
Use rabbit anti- SA2/STAG2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for SA2/STAG2 at approximately 141KD. The expected band size for SA2/STAG2 is at 141KD.|Figure 2. IHC analysis using anti- SA2/STAG2 antibody (A03624-1). detected in paraffin-embedded section of mouse brain tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 3. IHC analysis using anti- SA2/STAG2 antibody (A03624-1). detected in paraffin-embedded section of rat brain tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 4. IHC analysis using anti- SA2/STAG2 antibody (A03624-1). detected in paraffin-embedded section of human colon adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 5. IHC analysis using anti- SA2/STAG2 antibody (A03624-1). detected in paraffin-embedded section of human lung squamous cell carcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 6. IHC analysis using anti- SA2/STAG2 antibody (A03624-1). detected in paraffin-embedded section of human ovarian cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 7. IHC analysis using anti- SA2/STAG2 antibody (A03624-1). detected in paraffin-embedded section of human penis squamous cell carcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 8. IHC analysis using anti- SA2/STAG2 antibody (A03624-1). detected in paraffin-embedded section of human placenta tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 9. IHC analysis using anti- SA2/STAG2 antibody (A03624-1). detected in paraffin-embedded section of human liver cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 10. IHC analysis using anti- SA2/STAG2 antibody (A03624-1). detected in paraffin-embedded section of human colon cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 11. IF analysis of SA2/STAG2 using anti-SA2/STAG2 antibody (A03624-1).
SA2/STAG2 was detected in a paraffin-embedded section of human colon cancer tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).|Figure 12. ICC analysis using anti- SA2/STAG2 antibody (A03624-1) and anti-Tubulin alpha antibody (M03989-3). were detected in immersion fixed SiHa cell line. Cells were stained using the Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) and Dylight488-conjugated Anti- mouse IgG Secondary Antibody (green)(Catalog#BA1126).|Figure 13. Flow cytometry analysis of 293T cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 14. Flow cytometry analysis of U937 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
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Anti-SA2/STAG2 Antibody(A03624-1-100ul)
￥1960
