- ￥1960
- BOSTER
- A06953-1-100ul
- 中国
- 2025年07月08日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 技术资料
- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A06953-1 |
|---|---|
| 产品名称 | Anti-QARS1 Antibody |
| 基因名 | QARS1 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 免疫原 | E.coli-derived human QARS1 recombinant protein (Position: Q32-K774). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Glutaminyl-tRNA synthetase is an enzyme that in humans is encoded by the QARS gene. Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. In metazoans, 9 aminoacyl-tRNA synthetases specific for glutamine (gln), glutamic acid (glu), and 7 other amino acids are associated within a multienzyme complex. Although present in eukaryotes, glutaminyl-tRNA synthetase (QARS) is absent from many prokaryotes, mitochondria, and chloroplasts, in which Gln-tRNA(Gln) is formed by transamidation of the misacylated Glu-tRNA(Gln). Glutaminyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family. Alternative splicing results in multiple transcript variants. |
| 研究类别 | 1. Lamour, V., Quevillon, S., Diriong, S., N'Guyen, V. C., Lipinski, M., Mirande, M. Evolution of the Glx-tRNA synthetase family: the glutaminyl enzyme as a case of horizontal gene transfer. Proc. Nat. Acad. Sci. 91: 8670-8674, 1994.2. Lo, W.-S., Gardiner, E., Xu, Z., Lau, C.-F., Wang, F., Zhou, J. J., Mendlein, J. D., Nangle, L. A., Chiang, K. P., Yang, X.-L., Au, K.-F., Wong, W. H., Guo, M., Zhang, M., Schimmel, P. Human tRNA synthetase catalytic nulls with diverse functions. Science 345: 328-332, 2014.3. Zhang, X., Ling, J., Barcia, G., Jing, L., Wu, J., Barry, B. J., Mochida, G. H., Hill, R. S., Weimer, J. M., Stein, Q., Poduri, A., Partlow, J. N., and 13 others. Mutations in QARS, encoding glutaminyl-tRNA synthetase, cause progressive microcephaly, cerebral-cerebellar atrophy, and intractable seizures. Am. J. Hum. Genet. 94: 547-558, 2014. |
| Uniprot ID | QARS1: P47897 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| ELISA: | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
点击图片放大
[list_product_images]Figure 1. Western blot analysis of anti- QARS1 antibody (A06953-1). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: rat RH-35 whole cell lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
Use rabbit anti- QARS1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for QARS1 at approximately 88KD. The expected band size for QARS1 is at 88KD.|Figure 2. IHC analysis using anti- QARS1 antibody (A06953-1). detected in paraffin-embedded section of human breast cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 3. IHC analysis using anti- QARS1 antibody (A06953-1). detected in paraffin-embedded section of human glioblastoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 4. IHC analysis using anti- QARS1 antibody (A06953-1). detected in paraffin-embedded section of human testicular seminoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 5. IHC analysis using anti- QARS1 antibody (A06953-1). detected in paraffin-embedded section of human thyroid cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 6. ICC analysis using anti- QARS1 antibody (A06953-1). was detected in immersion fixed T-47D cell line. Cells were stained using the Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) and counterstained with DAPI (blue).|Figure 7. Flow cytometry analysis of THP-1 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: rat RH-35 whole cell lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
Use rabbit anti- QARS1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for QARS1 at approximately 88KD. The expected band size for QARS1 is at 88KD.|Figure 2. IHC analysis using anti- QARS1 antibody (A06953-1). detected in paraffin-embedded section of human breast cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 3. IHC analysis using anti- QARS1 antibody (A06953-1). detected in paraffin-embedded section of human glioblastoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 4. IHC analysis using anti- QARS1 antibody (A06953-1). detected in paraffin-embedded section of human testicular seminoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 5. IHC analysis using anti- QARS1 antibody (A06953-1). detected in paraffin-embedded section of human thyroid cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 6. ICC analysis using anti- QARS1 antibody (A06953-1). was detected in immersion fixed T-47D cell line. Cells were stained using the Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) and counterstained with DAPI (blue).|Figure 7. Flow cytometry analysis of THP-1 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
技术资料暂无技术资料 索取技术资料
Anti-QARS1 Antibody(A06953-1-100ul)
￥1960
