- ￥1960
- BOSTER
- A00896-1-100ul
- 中国
- 2025年06月25日
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大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A00896-1 |
|---|---|
| 产品名称 | Anti-LIN28B Antibody |
| 基因名 | LIN28B |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 免疫原 | E.coli-derived human LIN28B recombinant protein (Position: E23-K249). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | The protein encoded by this gene belongs to the lin-28 family, which is characterized by the presence of a cold-shock domain and a pair of CCHC zinc finger domains. This gene is highly expressed in testis, fetal liver, placenta, and in primary human tumors and cancer cell lines. It is negatively regulated by microRNAs that target sites in the 3' UTR, and overexpression of this gene in primary tumors is linked to the repression of let-7 family of microRNAs and derepression of let-7 targets, which facilitates cellular transformation. |
| 研究类别 | 1. Basak, A., Munschauer, M., Lareau, C. A., Montbleau, K. E., Ulirsch, J. C., Hartigan, C. R., Schenone, M., Lian, J., Wang, Y., Huang, Y., Wu, X., Gehrke, L., and 9 others. Control of human hemoglobin switching by LIN28B-mediated regulation of BCL11A translation. Nature Genet. 52: 138-145, 2020. 2. Guo, Y., Chen, Y., Ito, H., Watanabe, A., Ge, X., Kodama, T., Aburatani, H. Identification and characterization of lin-28 homolog B (LIN28B) in human hepatocellular carcinoma. Gene 384: 51-61, 2006. 3. Molenaar, J. J., Domingo-Fernandez, R., Ebus, M. E., Lindner, S., Koster, J., Drabek, K., Mestdagh, P., van Sluis, P., Valentijn, L. J., van Nes, J., Broekmans, M., Haneveld, F., and 18 others. LIN28B induces neuroblastoma and enhances MYCN levels via let-7 suppression. Nature Genet. 44: 1199-1206, 2012. |
| Uniprot ID | LIN28B: Q6ZN17 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| ELISA: | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- LIN28B antibody (A00896-1). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human HepG2 whole cell lysates.
Use rabbit anti- LIN28B 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for LIN28B at approximately 35KD. The expected band size for LIN28B is at 27KD.|Figure 2. IHC analysis using anti- LIN28B antibody (A00896-1). detected in paraffin-embedded section of human placenta tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.[/list_product_images]
Lane 1: human 293T whole cell lysates,
Lane 2: human HepG2 whole cell lysates.
Use rabbit anti- LIN28B 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for LIN28B at approximately 35KD. The expected band size for LIN28B is at 27KD.|Figure 2. IHC analysis using anti- LIN28B antibody (A00896-1). detected in paraffin-embedded section of human placenta tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.[/list_product_images]
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Anti-LIN28B Antibody(A00896-1-100ul)
￥1960
