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- 详细信息
- 技术资料
- 库存:
大量
- 供应商:
广州威佳科技有限公司
- 规格:
100ul
产品概况
| 货号 | MA00634 |
|---|---|
| 产品名称 | Anti-OPN/SPP1 Antibody (Clone#OTI6C12) |
| 基因名 | SPP1 |
| 抗体来源 | Mouse |
| 克隆 | OTI6C12 |
| 抗体亚型 | Mouse IgG1 |
| 分子量 | 33.7KD |
| 免疫原 | Human recombinant protein fragment corresponding to amino acids 17-314 of human SPP1(NP_001035147) produced in E.coli. |
| 内容 | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
| 纯化方式 | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | Stable for 12 months from date of receipt. Store at -20°C as received. |
| 背景资料 | The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| 研究类别 | 1. "Entrez Gene: SPP1 secreted phosphoprotein 1". 2. Reinholt FP, Hultenby K, Oldberg A, Heineg rd D (June 1990). "Osteopontin--a possible anchor of osteoclasts to bone". Proc. Natl. Acad. Sci. U.S.A. 87 (12): 4473–5.3. Wang KX, Denhardt DT (2008). "Osteopontin: role in immune regulation and stress responses". Cytokine Growth Factor Rev. 19 (5-6): 333–45. |
| Uniprot ID | SPP1: P10451 |
产品应用细节
为了提供优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:2000 |
*稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPP1. [/list_product_images]
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Anti-OPN/SPP1 Antibody (Clone#OTI6C12)(MA00634-100ul)
¥1960







