- ￥1960
- BOSTER
- MA01106-100ul
- 中国
- 2025年07月11日
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- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | MA01106 |
|---|---|
| 产品名称 | Anti-ARG1 Antibody (Clone#OTI4E6) |
| 基因名 | ARG1 |
| 抗体来源 | Mouse |
| 克隆 | OTI4E6 |
| 抗体亚型 | Mouse IgG1 |
| 分子量 | 34.6KD |
| 免疫原 | Full length human recombinant protein of human ARG1 (NP_000036) produced in HEK293T cell. |
| 内容 | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
| 纯化方式 | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | Stable for 12 months from date of receipt. Store at -20°C as received. |
| 背景资料 | Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. [provided by RefSeq] |
| 研究类别 | 1. Cardoso, M. L., Martins, E., Vasconcelos, R., Vilarinho, L., Rocha, J.Identification of a novel R21X mutation in the liver-type arginase gene (ARG1) in four Portuguese patients with argininemia.Hum. Mutat. 14: 355-356, 1999.2. Grody, W. W., Dodson, A., Klein, D., Kern, R. M., Bassand, P., Cederbaum, S. D.Molecular genetic study of human arginase deficiency. (Abstract)Am. J. Hum. Genet. 45 (suppl.): A191 only, 1989.3. Haraguchi, Y., Takiguchi, M., Amaya, Y., Kawamoto, S., Matsuda, I., Mori, M.Molecular cloning and nucleotide sequence of cDNA for human liver arginase.Proc. Nat. Acad. Sci. 84: 412-415, 1987. |
| Uniprot ID | ARG1: P05089 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:1000~2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50 |
| Flow cytometry (FCM): | 1:100 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ARG1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA01106)|Figure 2. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARG1 monoclonal antibody.|Figure 3. HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ARG1 antibody, and then analyzed by flow cytometry.|Figure 4. Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ARG1 monoclonal antibody (1:200).|Figure 5. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARG1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARG1(Cat# MA01106). [/list_product_images]
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Anti-ARG1 Antibody (Clone#OTI4E6)(MA01106-100ul)
￥1960
