- ￥1960
- BOSTER
- MA02632-100ul
- 中国
- 2025年07月12日
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- 技术资料
- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | MA02632 |
|---|---|
| 产品名称 | Anti-CDH5 Antibody (Clone#OTI1F4) |
| 基因名 | CDH5 |
| 抗体来源 | Mouse |
| 克隆 | OTI1F4 |
| 抗体亚型 | Mouse IgG1 |
| 免疫原 | Human recombinant protein fragment corresponding to amino acids 48-333 of human CDH5 (NP_001786) produced in E.coli. |
| 内容 | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
| 纯化方式 | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | Stable for 12 months from date of receipt. Store at -20°C as received. |
| 背景资料 | CDH5(Cadherin 5), also known as VE-cadherin, is a type of cadherin. It is encoded by the human gene CDH5. Kremmidiotis et al.(1998) mapped the human CDH5 gene to 16q22.1 using somatic cell hybrid panels. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. Therefore it was concluded that VE-cadherin serves the purpose of maintaining newly formed vessels. |
| 研究类别 | 1. Huber, P., Dalmon, J., Engiles, J., Breviario, F., Gory, S., Siracusa, L. D., Buchberg, A. M., Dejana, E. Genomic structure and chromosomal mapping of the mouse VE-cadherin gene (Cdh5). Genomics 32: 21-28, 1996.2. Kremmidiotis, G., Baker, E., Crawford, J., Eyre, H. J., Nahmias, J., Callen, D. F. Localization of human cadherin genes to chromosome regions exhibiting cancer-related loss of heterozygosity. Genomics 49: 467-471, 1998. |
| Uniprot ID | CDH5: P33151 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:150 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CDH5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, MA02632)|Figure 2. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDH5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDH5(Cat# MA02632).[/list_product_images]
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Anti-CDH5 Antibody (Clone#OTI1F4)(MA02632-100ul)
￥1960
