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- 详细信息
- 技术资料
- 库存:
大量
- 供应商:
广州威佳科技有限公司
- 规格:
100ul
产品概况
| 货号 | MA02686 |
|---|---|
| 产品名称 | Anti-HPGD Antibody (Clone#OTI1C10) |
| 基因名 | HPGD |
| 抗体来源 | Mouse |
| 克隆 | OTI1C10 |
| 抗体亚型 | Mouse IgG1 |
| 分子量 | 28.8KD |
| 免疫原 | Full length human recombinant protein of human HPGD (NP_000851) produced in HEK293T cell. |
| 内容 | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
| 纯化方式 | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | Stable for 12 months from date of receipt. Store at -20°C as received. |
| 背景资料 | This gene encodes a member of the short-chain nonmetalloenzyme alcohol dehydrogenase protein family. The encoded enzyme is responsible for the metabolism of prostaglandins, which function in a variety of physiologic and cellular processes such as inflammation. Mutations in this gene result in primary autosomal recessive hypertrophic osteoarthropathy and cranioosteoarthropathy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
| 研究类别 | 1. Coggins, K. G., Latour, A., Nguyen, M. S., Audoly, L., Coffman, T. M., Koller, B. H. Metabolism of PGE2 by prostaglandin dehydrogenase is essential for remodeling the ductus arteriosus. (Letter) Nature Med. 8: 91-92, 2002. 2. Dabir, T., Sills, A. M., Hall, C. M., Bennett, C., Wilson, L. C., Hennekam, R. C. M. Cranio-osteoarthropathy in sibs. Clin. Dysmorph. 16: 197-201, 2007. 3. Ensor, C. M., Yang, J. Y., Okita, R. T., Tai, H. H. Cloning and sequence analysis of the cDNA for human placental NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase. J. Biol. Chem. 265: 14888-14891, 1990. |
| Uniprot ID | HPGD: P15428 |
产品应用细节
为了提供优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:150 |
| Flow cytometry (FCM): | 1:100 |
*稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HPGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA02686)|Figure 2. HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HPGD antibody, and then analyzed by flow cytometry.|Figure 3. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HPGD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HPGD. [/list_product_images]
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Anti-HPGD Antibody (Clone#OTI1C10)(MA02686-100ul)
¥1960







