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- 详细信息
- 技术资料
- 库存:
大量
- 供应商:
广州威佳科技有限公司
- 规格:
100ul
产品概况
| 货号 | MA06921 |
|---|---|
| 产品名称 | Anti-TRIP13 Antibody (Clone#OTI2F5) |
| 基因名 | TRIP13 |
| 抗体来源 | Mouse |
| 克隆 | OTI2F5 |
| 抗体亚型 | Mouse IgG1 |
| 免疫原 | Human recombinant protein fragment corresponding to amino acids 31-148 of human TRIP13(NP_001159732) produced in E.coli. |
| 内容 | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
| 纯化方式 | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | Stable for 12 months from date of receipt. Store at -20°C as received. |
| 背景资料 | This gene encodes a protein that interacts with thyroid hormone receptors, also known as hormone-dependent transcription factors. The gene product interacts specifically with the ligand binding domain. This gene is one of several that may play a role in early-stage non-small cell lung cancer. [provided by RefSeq, Oct 2009] |
| 研究类别 | 1. Alfieri, C., Chang, L., Barford, D. Mechanism for remodelling of the cell cycle checkpoint protein MAD2 by the ATPase TRIP13. Nature 559: 274-278, 2018. 2. Eytan, E., Wang, K., Miniowitz-Shemtov, S., Sitry-Shevah, D., Kaisari, S., Yen, T. J., Liu, S.-T., Hershko, A. Disassembly of mitotic checkpoint complexes by the joint action of the AAA-ATPase TRIP13 and p13(comet). Proc. Nat. Acad. Sci. 111: 12019-12024, 2014.3. Gross, M. B. Personal Communication. Baltimore, Md. 6/25/2014. |
| Uniprot ID | TRIP13: Q15645 |
产品应用细节
为了提供优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500~2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:2000 |
*稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT) and TRIP13-Knockout 293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-TRIP13 monoclonal antibody MA06921, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody ([MA01692]) as a loading control.|Figure 2. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TRIP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA06921) (1:2000)|Figure 3. Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-TRIP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA06921) (1:2000)|Figure 4. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TRIP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA06921) (1:2000)|Figure 5. Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-TRIP13 monoclonal antibody (293T: human; HeLa: human; Jurkat: human;) (1:500).|Figure 6. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIP13 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIP13 (Cat# MA06921)(1:2000).[/list_product_images]
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Anti-TRIP13 Antibody (Clone#OTI2F5)(MA06921-100ul)
¥1960







