Anti-TRIP13 Antibody (Clone#OTI2F5)(MA06921-100ul)

Anti-TRIP13 Antibody (Clone#OT

I2F5)(MA06921-100ul)
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  • ¥1960
  • BOSTER已认证
  • MA06921-100ul
  • 中国
  • 2025年07月14日
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    • 详细信息
    • 技术资料
    • 库存

      大量

    • 供应商

      广州威佳科技有限公司

    • 规格

      100ul

    产品概况

    货号 MA06921
    产品名称 Anti-TRIP13 Antibody (Clone#OTI2F5)
    基因名 TRIP13
    抗体来源 Mouse
    克隆 OTI2F5
    抗体亚型 Mouse IgG1
    免疫原 Human recombinant protein fragment corresponding to amino acids 31-148 of human TRIP13(NP_001159732) produced in E.coli.
    内容 PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
    纯化方式 Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
    浓度 500 ug/ml
    产品形态 Liquid
    保存条件 Stable for 12 months from date of receipt. Store at -20°C as received.
    背景资料 This gene encodes a protein that interacts with thyroid hormone receptors, also known as hormone-dependent transcription factors. The gene product interacts specifically with the ligand binding domain. This gene is one of several that may play a role in early-stage non-small cell lung cancer. [provided by RefSeq, Oct 2009]
    研究类别 1. Alfieri, C., Chang, L., Barford, D. Mechanism for remodelling of the cell cycle checkpoint protein MAD2 by the ATPase TRIP13. Nature 559: 274-278, 2018. 2. Eytan, E., Wang, K., Miniowitz-Shemtov, S., Sitry-Shevah, D., Kaisari, S., Yen, T. J., Liu, S.-T., Hershko, A. Disassembly of mitotic checkpoint complexes by the joint action of the AAA-ATPase TRIP13 and p13(comet). Proc. Nat. Acad. Sci. 111: 12019-12024, 2014.3. Gross, M. B. Personal Communication. Baltimore, Md. 6/25/2014.
    Uniprot ID TRIP13: Q15645

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    [list_product_images]Figure 1. Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT) and TRIP13-Knockout 293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-TRIP13 monoclonal antibody MA06921, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody ([MA01692]) as a loading control.|Figure 2. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TRIP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA06921) (1:2000)|Figure 3. Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-TRIP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA06921) (1:2000)|Figure 4. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TRIP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA06921) (1:2000)|Figure 5. Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-TRIP13 monoclonal antibody (293T: human; HeLa: human; Jurkat: human;) (1:500).|Figure 6. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIP13 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIP13 (Cat# MA06921)(1:2000).[/list_product_images]

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