- ￥1960
- BOSTER
- A01495-2-100ul
- 中国
- 2025年07月10日
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广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A01495-2 |
|---|---|
| 产品名称 | Anti-HAX1 Antibody |
| 基因名 | HBA1/HBA2 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 36KD |
| 免疫原 | E.coli-derived human HAX1 recombinant protein (Position: M1-R279). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | HCLS1-associated protein X-1 is a protein that in humans is encoded by the HAX1 gene. The protein encoded by this gene is known to associate with hematopoietic cell-specific Lyn substrate 1, a substrate of Src family tyrosine kinases. It also interacts with the product of the polycystic kidney disease 2 gene, mutations in which are associated with autosomal-dominant polycystic kidney disease, and with the F-actin-binding protein, cortactin. It was earlier thought that this gene product is mainly localized in the mitochondria, however, recent studies indicate it to be localized in the cell body. Mutations in this gene result in autosomal recessive severe congenital neutropenia, also known as Kostmann disease. Two transcript variants encoding different isoforms have been found for this gene. |
| 研究类别 | 1. Carlsson, G., Fasth, A. Infantile genetic agranulocytosis, morbus Kostmann: presentation of six cases from the original 'Kostmann family' and a review. Acta Paediat. 90: 757-764, 2001. 2. Chao, J.-R., Parganas, E., Boyd, K., Hong, C. Y., Opferman, J. T., Ihle, J. N. Hax1-mediated processing of HtrA2 by Parl allows survival of lymphocytes and neurons. Nature 452: 98-102, 2008. Note: Erratum: Nature 452: 900 only, 2008. 3. Dale, D. C., Person, R. E., Bolyard, A. A., Aprikyan, A. G., Bos, C., Bonilla, M. A., Boxer, L. A., Kannourakis, G., Zeidler, C., Welte, K., Benson, K. F., Horwitz, M. Mutations in the gene encoding neutrophil elastase in congenital and cyclic neutropenia. Blood 96: 2317-2322, 2000. |
| Uniprot ID | HBA1/HBA2: O00165 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Flow Cytometry (FCM): | 1-3 μg/1x106 cells |
| ELISA: | 1:100-1000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti-HAX1 antibody (A01495-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: MOLT-4 whole cell lysates,
Lane 2: Raji whole cell lysates,
Lane 3: 293T whole cell lysates,
Lane 4: Hela whole cell lysates,
Lane 5: THP-1 whole cell lysates,
Lane 6: MCF-7 whole cell lysates,
Lane 7: Jurkat whole cell lysates,
Lane 8: PC-3 whole cell lysates.
Use mouse anti-HAX1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for HAX1 at approximately 36KD. The expected band size for HAX1 is at 32KD.|Figure 2. IHC analysis using anti-HAX1 antibody (A01495-2). detected in paraffin-embedded section of human esophageal squamous cell carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis using anti- HAX1 antibody (A01495-2). detected in paraffin-embedded section of human Colorectal adenocarcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4. IHC analysis using anti- HAX1 antibody (A01495-2). detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 5. Flow cytometry analysis of HEL cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: MOLT-4 whole cell lysates,
Lane 2: Raji whole cell lysates,
Lane 3: 293T whole cell lysates,
Lane 4: Hela whole cell lysates,
Lane 5: THP-1 whole cell lysates,
Lane 6: MCF-7 whole cell lysates,
Lane 7: Jurkat whole cell lysates,
Lane 8: PC-3 whole cell lysates.
Use mouse anti-HAX1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for HAX1 at approximately 36KD. The expected band size for HAX1 is at 32KD.|Figure 2. IHC analysis using anti-HAX1 antibody (A01495-2). detected in paraffin-embedded section of human esophageal squamous cell carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis using anti- HAX1 antibody (A01495-2). detected in paraffin-embedded section of human Colorectal adenocarcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4. IHC analysis using anti- HAX1 antibody (A01495-2). detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 5. Flow cytometry analysis of HEL cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-HAX1 Antibody(A01495-2-100ul)
￥1960
