- ￥1960
- BOSTER
- M04803-1-100ul
- 中国
- 2025年07月10日
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- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | M04803-1 |
|---|---|
| 产品名称 | Anti-MDH2 Antibody (monoclonal, 5D8C1) |
| 基因名 | MDH2 |
| 抗体来源 | Mouse |
| 克隆 | Monoclonal |
| 抗体亚型 | Mouse IgG2b |
| 分子量 | 36KD |
| 免疫原 | E.coli-derived human MDH2 recombinant protein (Position: A9-L223). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Malate dehydrogenase, mitochondrial also known as malate dehydrogenase 2 is an enzyme that in humans is encoded by the MDH2 gene. Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Several transcript variants encoding different isoforms have been found for this gene. |
| 研究类别 | 1. Ait-El-Mkadem, S., Dayem-Quere, M., Gusic, M., Chaussenot, A., Bannwarth, S., Francois, B., Genin, E. C., Fragaki, K., Volker-Touw, C. L. M., Vasnier, C., Serre, V., van Gassen, K. L. I., and 24 others. Mutations in MDH2, encoding a Krebs cycle enzyme, cause early-onset severe encephalopathy. Am. J. Hum. Genet. 100: 151-159, 2017. 2. Benn, P., Chern, C. J., Bruns, G., Craig, I. W., Croce, C. M. Assignment of the genes for human beta-glucuronidase and mitochondrial malate dehydrogenase to the region pter-q22 of chromosome 7. Cytogenet. Cell Genet. 19: 273-280, 1977. 3. Blake, N. M. Malate dehydrogenase types in the Asian-Pacific area, and a description of new phenotypes. Hum. Genet. 43: 69-80, 1978. |
| Uniprot ID | MDH2: P40926 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence(ICC/IF): | 1:50-400 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of anti- MDH2 antibody (M04803-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: U-87MG whole cell lysates,
Lane 3: A431 whole cell lysates.
Use mouse anti- MDH2 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for MDH2 at approximately 36KD. The expected band size for MDH2 is at 36KD.|Figure 2. IHC analysis using anti- MDH2 antibody (M04803-1). detected in paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 3. IHC analysis using anti- MDH2 antibody (M04803-1). detected in paraffin-embedded section of human cervical cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 4. IHC analysis using anti- MDH2 antibody (M04803-1). detected in paraffin-embedded section of human squamous cell carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 5. ICC analysis using anti- MDH2 antibody (M04803-1). was detected in immersion fixed Hela cell. Cells were stained using the cy3-conjugated Anti- mouse IgG Secondary Antibody (red)(Catalog # BA1031) and counterstained with DAPI (blue)[/list_product_images]
Lane 1: Hela whole cell lysates,
Lane 2: U-87MG whole cell lysates,
Lane 3: A431 whole cell lysates.
Use mouse anti- MDH2 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for MDH2 at approximately 36KD. The expected band size for MDH2 is at 36KD.|Figure 2. IHC analysis using anti- MDH2 antibody (M04803-1). detected in paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 3. IHC analysis using anti- MDH2 antibody (M04803-1). detected in paraffin-embedded section of human cervical cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 4. IHC analysis using anti- MDH2 antibody (M04803-1). detected in paraffin-embedded section of human squamous cell carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 5. ICC analysis using anti- MDH2 antibody (M04803-1). was detected in immersion fixed Hela cell. Cells were stained using the cy3-conjugated Anti- mouse IgG Secondary Antibody (red)(Catalog # BA1031) and counterstained with DAPI (blue)[/list_product_images]
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Anti-MDH2 Antibody (monoclonal, 5D8C1)(M04803-1-100ul)
￥1960
