- ￥1960
- BOSTER
- A06381-1-100ul
- 中国
- 2025年07月09日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A06381-1 |
|---|---|
| 产品名称 | Anti-SAP18 Antibody |
| 基因名 | SAP18 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 18KD |
| 免疫原 | E.coli-derived human SAP18 recombinant protein (Position: M1-Y153). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Histone deacetylase complex subunit SAP18 is an enzyme that in humans is encoded by the SAP18 gene. Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP30, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This protein directly interacts with SIN3 and enhances SIN3-mediated transcriptional repression when tethered to the promoter. A pseudogene has been identified on chromosome 2. |
| 研究类别 | 1. Zhang, Y., Iratni, R., Erdjument-Bromage, H., Tempst, P., Reinberg, D. Histone deacetylases and SAP18, a novel polypeptide, are components of a human Sin3 complex. Cell 89: 357-364, 1997. |
| Uniprot ID | SAP18: O00422 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| ELISA: | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- SAP18 antibody (A06381-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: rat RH-35 whole cell lysates,
Lane 5: mouse NIH/3T3 whole cell lysates.
Use rabbit anti- SAP18 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for SAP18 at approximately 18KD. The expected band size for SAP18 is at 18KD.|Figure 2. IHC analysis using anti- SAP18 antibody (A06381-1). detected in paraffin-embedded section of human duodenal adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.|Figure 3. IHC analysis using anti- SAP18 antibody (A06381-1). detected in paraffin-embedded section of human esophageal squamous carcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.|Figure 4. IHC analysis using anti- SAP18 antibody (A06381-1). detected in paraffin-embedded section of human liver cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.|Figure 5. IF analysis using anti- SAP18 antibody (A06381-1). detected in paraffin-embedded section of human esophageal squamous carcinoma tissue. The tissue section were stained using the Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog # BA1135).|Figure 6. IF analysis using anti- SAP18 antibody (A06381-1). detected in paraffin-embedded section of human ovarian cancer tissue. The tissue section were stained using the Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog # BA1135).|Figure 7. ICC analysis using anti- SAP18 antibody (A06381-1) and anti-Tubulin alpha antibody (M03989-3). were detected in immersion fixed MCF-7 cell line. Cells were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) and Dylight488-conjugated Anti- mouse IgG Secondary Antibody (green)(Catalog # BA1126).|Figure 8. Flow cytometry analysis of U20S cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: rat RH-35 whole cell lysates,
Lane 5: mouse NIH/3T3 whole cell lysates.
Use rabbit anti- SAP18 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for SAP18 at approximately 18KD. The expected band size for SAP18 is at 18KD.|Figure 2. IHC analysis using anti- SAP18 antibody (A06381-1). detected in paraffin-embedded section of human duodenal adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.|Figure 3. IHC analysis using anti- SAP18 antibody (A06381-1). detected in paraffin-embedded section of human esophageal squamous carcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.|Figure 4. IHC analysis using anti- SAP18 antibody (A06381-1). detected in paraffin-embedded section of human liver cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.|Figure 5. IF analysis using anti- SAP18 antibody (A06381-1). detected in paraffin-embedded section of human esophageal squamous carcinoma tissue. The tissue section were stained using the Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog # BA1135).|Figure 6. IF analysis using anti- SAP18 antibody (A06381-1). detected in paraffin-embedded section of human ovarian cancer tissue. The tissue section were stained using the Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog # BA1135).|Figure 7. ICC analysis using anti- SAP18 antibody (A06381-1) and anti-Tubulin alpha antibody (M03989-3). were detected in immersion fixed MCF-7 cell line. Cells were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) and Dylight488-conjugated Anti- mouse IgG Secondary Antibody (green)(Catalog # BA1126).|Figure 8. Flow cytometry analysis of U20S cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-SAP18 Antibody(A06381-1-100ul)
￥1960
