- ￥1960
- BOSTER
- A02334-1-100ul
- 中国
- 2025年07月10日
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- 技术资料
- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A02334-1 |
|---|---|
| 产品名称 | Anti-ACK1/TNK2 Antibody |
| 基因名 | TNK2 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 115KD |
| 免疫原 | E.coli-derived human TNK2/ACK1 recombinant protein (Position: M491-R1038). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Activated CDC42 kinase 1, also known as ACK1, is an enzyme that in humans is encoded by the TNK2 gene. This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined. |
| 研究类别 | 1. Hitomi, Y., Heinzen, E. L., Donatello, S., Dahl, H.-H., Damiano, J. A., McMahon, J. M., Berkovic, S. F., Scheffer, I. E., Legros, B., Rai, M., Weckhuysen, S., Suls, A., De Jonghe, P., Pandolfo, M., Goldstein, D. B., Van Bogaert, P., Depondt, C. Mutations in TNK2 in severe autosomal recessive infantile onset epilepsy. Ann. Neurol. 74: 496-501, 2013.2. La Torre, A., del Rio, J. A., Soriano, E., Urena, J. M. Expression pattern of ACK1 tyrosine kinase during brain development in the mouse. Gene Expr. Patterns 6: 886-892, 2006. 3. Manser, E., Leung, T., Salihuddin, H., Tan, L., Lim, L. A non-receptor tyrosine kinase that inhibits the GTPase activity of p21cdc42. Nature 363: 364-367, 1993. |
| Uniprot ID | TNK2: Q07912 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| ELISA: | 1:100-1000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- ACK1/TNK2 antibody (A02334-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human HepG2 whole cell lysates.
Use rabbit anti- ACK1/TNK2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ACK1/TNK2 at approximately 115KD. The expected band size for ACK1/TNK2 is at 115KD.|Figure 2. Flow cytometry analysis of U20S cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human HepG2 whole cell lysates.
Use rabbit anti- ACK1/TNK2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ACK1/TNK2 at approximately 115KD. The expected band size for ACK1/TNK2 is at 115KD.|Figure 2. Flow cytometry analysis of U20S cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-ACK1/TNK2 Antibody(A02334-1-100ul)
￥1960
