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- BOSTER
- A02009-3-100ul
- 中国
- 2025年07月12日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A02009-3 |
|---|---|
| 产品名称 | Anti-TWEAK/Tnfsf12 Antibody |
| 基因名 | Tnfsf12 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 27KD |
| 免疫原 | E.coli-derived mouse TWEAK/Tnfsf12 recombinant protein (Position: S46-H249). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Tumor necrosis factor ligand superfamily member 12 also known as TNF-related weak inducer of apoptosis (TWEAK) is a protein that in humans is encoded by the TNFSF12 gene. Predicted to enable receptor ligand activity. Acts upstream of or within extrinsic apoptotic signaling pathway. Located in extracellular region. Is expressed in Meckel's cartilage; hip; skeleton; and superior cervical ganglion. Orthologous to several human genes including TNFSF12-TNFSF13 (TNFSF12-TNFSF13 readthrough). |
| 研究类别 | 1. Chicheportiche, Y., Bourdon, P. R., Xu, H., Hsu, Y.-M., Scott, H., Hession, C., Garcia, I., Browning, J. L. TWEAK, a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis. J. Biol. Chem. 272: 32401-32410, 1997. 2. Jain, M., Jakubowski, A., Cui, L., Shi, J., Su, L., Bauer, M., Guan, J., Lim, C. C., Naito, Y., Thompson, J. S., Sam, F., Ambrose, C., and 9 others. A novel role for tumor necrosis factor-like weak inducer of apoptosis (TWEAK) in the development of cardiac dysfunction and failure. Circulation 119: 2058-2068, 2009. 3. Kaptein, A., Jansen, M., Dilaver, G., Kitson, J., Dash, L., Wang, E., Owen, M. J., Bodmer, J.-L., Tschopp, J., Farrow, S. N. Studies on the interaction between TWEAK and the death receptor WSL-1/TRAMP (DR3). FEBS Lett. 485: 135-141, 2000. |
| Uniprot ID | Tnfsf12: O54907 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| ELISA: | 1:100-1000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- TWEAK/Tnfsf12 antibody (A02009-3). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: rat skeletal muscle tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: mouse heart tissue lysates.
Use rabbit anti- TWEAK/Tnfsf12 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for TWEAK/Tnfsf12 at approximately 27KD. The expected band size for TWEAK/Tnfsf12 is at 22KD.[/list_product_images]
Lane 1: rat skeletal muscle tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: mouse heart tissue lysates.
Use rabbit anti- TWEAK/Tnfsf12 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for TWEAK/Tnfsf12 at approximately 27KD. The expected band size for TWEAK/Tnfsf12 is at 22KD.[/list_product_images]
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Anti-TWEAK/Tnfsf12 Antibody(A02009-3-100ul)
￥1960
