- ￥1960
- BOSTER
- M01451-3-100ul
- 中国
- 2025年07月14日
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- 规格：
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产品概况
| 货号 | M01451-3 |
|---|---|
| 产品名称 | Anti-Annexin A1/ANXA1 Antibody (monoclonal, 6B7F8) |
| 基因名 | ANXA1 |
| 抗体来源 | Mouse |
| 克隆 | Monoclonal(Clone:6B7F8) |
| 抗体亚型 | Mouse IgG2b |
| 分子量 | 35-39KD |
| 免疫原 | E.coli-derived human Annexin A1 recombinant protein (Position: A2-N346). Human Annexin A1 shares 88% and 89% amino acid (aa) sequence identity with mouse and rat Annexin A1, respectively. |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | ANXA1, also known as lipocortin I or Annexin A1, is a protein that in humans is encoded by the ANXA1 gene. It is mapped to 9q21.13. ANXA1 belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. ANXA1 protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Lower peptide concentrations possibly found in inflammatory situations elicit Ca(2+) transients without fully activating the mitogen-activated protein kinase pathway. This causes a specific inhibition of the transendothelial migration of neutrophils and a desensitization of neutrophils toward a chemoattractant challenge. These findings identified ANXA1 peptides as novel, endogenous FPR ligands and established a mechanistic basis of ANXA1-mediated antiinflammatory effects. |
| 研究类别 | 1. Wallner BP, Mattaliano RJ, Hession C, Cate RL, Tizard R, Sinclair LK, Foeller C, Chow EP, Browing JL, Ramachandran KL (1986). "Cloning and expression of human lipocortin, a phospholipase A2 inhibitor with potential anti-inflammatory activity". Nature 320 (6057): 77–81.2. "Entrez Gene: ANXA1 annexin A1".3. Walther, A., Riehemann, K., Gerke, V. A novel ligand of the formyl peptide receptor: annexin I regulates neutrophil extravasation by interacting with the FPR. Molec. Cell 5: 831-840, 2000. |
| Uniprot ID | ANXA1: P04083 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P). |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of anti- Annexin A1/ANXA1 antibody (M01451-3). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human U-87 MG whole cell lysates.
Use mouse anti- Annexin A1/ANXA1 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for Annexin A1/ANXA1 at approximately 35-39KD. The expected band size for Annexin A1/ANXA1 is at 39KD.|Figure 2. IHC analysis using anti- Annexin A1/ANXA1 antibody (M01451-3). detected in paraffin-embedded section of human colorectal adenocarcinoma tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.|Figure 3. IHC analysis using anti- Annexin A1/ANXA1 antibody (M01451-3). detected in paraffin-embedded section of human lung squamous cell carcinoma tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.|Figure 4. IHC analysis using anti- Annexin A1/ANXA1 antibody (M01451-3). detected in paraffin-embedded section of human placenta tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.|Figure 5. Flow cytometry analysis of A549 cell (1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: human A549 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human U-87 MG whole cell lysates.
Use mouse anti- Annexin A1/ANXA1 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for Annexin A1/ANXA1 at approximately 35-39KD. The expected band size for Annexin A1/ANXA1 is at 39KD.|Figure 2. IHC analysis using anti- Annexin A1/ANXA1 antibody (M01451-3). detected in paraffin-embedded section of human colorectal adenocarcinoma tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.|Figure 3. IHC analysis using anti- Annexin A1/ANXA1 antibody (M01451-3). detected in paraffin-embedded section of human lung squamous cell carcinoma tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.|Figure 4. IHC analysis using anti- Annexin A1/ANXA1 antibody (M01451-3). detected in paraffin-embedded section of human placenta tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.|Figure 5. Flow cytometry analysis of A549 cell (1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-Annexin A1/ANXA1 Antibody (monoclonal, 6B7F8)(M01451-3-100ul)
￥1960
