- ￥1960
- BOSTER
- A01888-3-100ul
- 中国
- 2025年07月13日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A01888-3 |
|---|---|
| 产品名称 | Anti-SMARCA2/BRM Antibody |
| 基因名 | SMARCA2 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 210KD |
| 免疫原 | E.coli-derived human SMARCA2/BRM recombinant protein (Position: V53-K1405). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Probable global transcription activator SNF2L2 is a protein that in humans is encoded by the SMARCA2 gene. It is mapped to 9p24.3. The protein encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism. |
| 研究类别 | 1. de la Serna, I. L., Carlson, K. A., Imbalzano, A. N. Mammalian SWI/SNF complexes promote MyoD-mediated muscle differentiation. Nature Genet. 27: 187-190, 2001. 2. Hakimi, M.-A., Bochar, D. A., Schmiesing, J. A., Dong, Y., Barak, O. G., Speicher, D. W., Yokomori, K., Shiekhattar, R. A chromatin remodelling complex that loads cohesin onto human chromosomes. Nature 418: 994-998, 2002. |
| Uniprot ID | SMARCA2: P51531 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| ELISA: | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of anti- SMARCA2/BRM antibody (A01888-3). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human U20S whole cell lysates,
Lane 2: rat PC-12 whole cell lysates,
Lane 3: mouse RAW264.7 whole cell lysates.
Use rabbit anti- SMARCA2/BRM 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for SMARCA2/BRM at approximately 210KD. The expected band size for SMARCA2/BRM is at 181KD.|Figure 2. IHC analysis using anti- SMARCA2/BRM antibody (A01888-3). detected in paraffin-embedded section of human appendix adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 3. IHC analysis using anti- SMARCA2/BRM antibody (A01888-3). detected in paraffin-embedded section of human breast cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 4. IHC analysis using anti- SMARCA2/BRM antibody (A01888-3). detected in paraffin-embedded section of human colon adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 5. IHC analysis using anti- SMARCA2/BRM antibody (A01888-3). detected in paraffin-embedded section of human diffuse large B cell lymphoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 6. IHC analysis using anti- SMARCA2/BRM antibody (A01888-3). detected in paraffin-embedded section of human esophageal squamous carcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 7. IHC analysis using anti- SMARCA2/BRM antibody (A01888-3). detected in paraffin-embedded section of human glioblastoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 8. IHC analysis using anti- SMARCA2/BRM antibody (A01888-3). detected in paraffin-embedded section of human placenta tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 9. IHC analysis using anti- SMARCA2/BRM antibody (A01888-3). detected in paraffin-embedded section of human prostate adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 10. IHC analysis using anti- SMARCA2/BRM antibody (A01888-3). detected in paraffin-embedded section of human right renal oncocytoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 11. IHC analysis using anti- SMARCA2/BRM antibody (A01888-3). detected in paraffin-embedded section of human spleen tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 12. IHC analysis using anti- SMARCA2/BRM antibody (A01888-3). detected in paraffin-embedded section of human thyroid papillary carcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 13. IHC analysis using anti- SMARCA2/BRM antibody (A01888-3). detected in paraffin-embedded section of human urothelial carcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 14. ICC analysis using anti- SMARCA2/BRM antibody (A01888-3) and anti-Tubulin beta antibody (M05613-4). were detected in immersion fixed A549 cell line. Cells were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) and Dylight488-conjugated Anti- mouse IgG Secondary Antibody (green)(Catalog#BA1126).|Figure 15. Flow cytometry analysis of THP-1 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: human U20S whole cell lysates,
Lane 2: rat PC-12 whole cell lysates,
Lane 3: mouse RAW264.7 whole cell lysates.
Use rabbit anti- SMARCA2/BRM 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for SMARCA2/BRM at approximately 210KD. The expected band size for SMARCA2/BRM is at 181KD.|Figure 2. IHC analysis using anti- SMARCA2/BRM antibody (A01888-3). detected in paraffin-embedded section of human appendix adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 3. IHC analysis using anti- SMARCA2/BRM antibody (A01888-3). detected in paraffin-embedded section of human breast cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 4. IHC analysis using anti- SMARCA2/BRM antibody (A01888-3). detected in paraffin-embedded section of human colon adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 5. IHC analysis using anti- SMARCA2/BRM antibody (A01888-3). detected in paraffin-embedded section of human diffuse large B cell lymphoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 6. IHC analysis using anti- SMARCA2/BRM antibody (A01888-3). detected in paraffin-embedded section of human esophageal squamous carcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 7. IHC analysis using anti- SMARCA2/BRM antibody (A01888-3). detected in paraffin-embedded section of human glioblastoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 8. IHC analysis using anti- SMARCA2/BRM antibody (A01888-3). detected in paraffin-embedded section of human placenta tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 9. IHC analysis using anti- SMARCA2/BRM antibody (A01888-3). detected in paraffin-embedded section of human prostate adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 10. IHC analysis using anti- SMARCA2/BRM antibody (A01888-3). detected in paraffin-embedded section of human right renal oncocytoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 11. IHC analysis using anti- SMARCA2/BRM antibody (A01888-3). detected in paraffin-embedded section of human spleen tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 12. IHC analysis using anti- SMARCA2/BRM antibody (A01888-3). detected in paraffin-embedded section of human thyroid papillary carcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 13. IHC analysis using anti- SMARCA2/BRM antibody (A01888-3). detected in paraffin-embedded section of human urothelial carcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 14. ICC analysis using anti- SMARCA2/BRM antibody (A01888-3) and anti-Tubulin beta antibody (M05613-4). were detected in immersion fixed A549 cell line. Cells were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) and Dylight488-conjugated Anti- mouse IgG Secondary Antibody (green)(Catalog#BA1126).|Figure 15. Flow cytometry analysis of THP-1 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-SMARCA2/BRM Antibody(A01888-3-100ul)
￥1960
