- ￥1960
- BOSTER
- A12026-1-100ul
- 中国
- 2025年07月15日
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- 库存：
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A12026-1 |
|---|---|
| 产品名称 | Anti-SNRPD2 Antibody |
| 基因名 | SNRPD2 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 16KD |
| 免疫原 | E.coli-derived human SNRPD2 recombinant protein (Position: M1-K118). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Small nuclear ribonucleoprotein Sm D2 is a protein that in humans is encoded by the SNRPD2 gene. The protein encoded by this gene belongs to the small nuclear ribonucleoprotein core protein family. It is required for pre-mRNA splicing and small nuclear ribonucleoprotein biogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. |
| 研究类别 | 1. Lehmeier, T., Raker, V., Hermann, H., Luhrmann, R. cDNA cloning of the Sm proteins D2 and D3 from human small nuclear ribonucleoproteins: evidence for a direct D1-D2 interaction. Proc. Nat. Acad. Sci. 91: 12317-12321, 1994.2. Rokeach, L. A., Haselby, J. A., Hoch, S. O. Molecular cloning of a cDNA encoding the human Sm-D antigen. Proc. Nat. Acad. Sci. 85: 4832-4836, 1988. |
| Uniprot ID | SNRPD2: P62316 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| ELISA: | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- SNRPD2 antibody (A12026-1). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human HL-60 whole cell lysates,
Lane 3: human THP-1 whole cell lysates,
Lane 4: rat testis tissue lysates,
Lane 5: mouse testis tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
Use rabbit anti- SNRPD2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for SNRPD2 at approximately 16KD. The expected band size for SNRPD2 is at 14KD.|Figure 2. IHC analysis using anti- SNRPD2 antibody (A12026-1). detected in paraffin-embedded section of Diffuse large B-cell lymphoma of human intestine tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 3. IHC analysis using anti- SNRPD2 antibody (A12026-1). detected in paraffin-embedded section of human appendix adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 4. IHC analysis using anti- SNRPD2 antibody (A12026-1). detected in paraffin-embedded section of human breast cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 5. IHC analysis using anti- SNRPD2 antibody (A12026-1). detected in paraffin-embedded section of human colon adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 6. IHC analysis using anti- SNRPD2 antibody (A12026-1). detected in paraffin-embedded section of human endometrioid adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 7. IHC analysis using anti- SNRPD2 antibody (A12026-1). detected in paraffin-embedded section of human glioblastoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 8. IHC analysis using anti- SNRPD2 antibody (A12026-1). detected in paraffin-embedded section of human liver cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 9. IHC analysis using anti- SNRPD2 antibody (A12026-1). detected in paraffin-embedded section of human prostate adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 10. IHC analysis using anti- SNRPD2 antibody (A12026-1). detected in paraffin-embedded section of human right renal oncocytoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 11. IHC analysis using anti- SNRPD2 antibody (A12026-1). detected in paraffin-embedded section of human thyroid papillary carcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 12. IHC analysis using anti- SNRPD2 antibody (A12026-1). detected in paraffin-embedded section of human urothelial carcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 13. IF analysis using anti- SNRPD2 antibody (A12026-1). detected in paraffin-embedded section of human squamous carcinoma tissue. The tissue section were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) and counterstained with DAPI (blue).|Figure 14. IF analysis using anti- SNRPD2 antibody (A12026-1). detected in paraffin-embedded section of human breast cancer tissue. The tissue section were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) and counterstained with DAPI (blue).|Figure 15. ICC analysis using anti- SNRPD2 antibody (A12026-1) and anti-Tubulin beta antibody (M05613-4). were detected in immersion fixed SiHa cell line. Cells were stained using the Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) and Dylight488-conjugated Anti- mouse IgG Secondary Antibody (green)(Catalog#BA1126).|Figure 16. Flow cytometry analysis of Jurkat cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: human A549 whole cell lysates,
Lane 2: human HL-60 whole cell lysates,
Lane 3: human THP-1 whole cell lysates,
Lane 4: rat testis tissue lysates,
Lane 5: mouse testis tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
Use rabbit anti- SNRPD2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for SNRPD2 at approximately 16KD. The expected band size for SNRPD2 is at 14KD.|Figure 2. IHC analysis using anti- SNRPD2 antibody (A12026-1). detected in paraffin-embedded section of Diffuse large B-cell lymphoma of human intestine tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 3. IHC analysis using anti- SNRPD2 antibody (A12026-1). detected in paraffin-embedded section of human appendix adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 4. IHC analysis using anti- SNRPD2 antibody (A12026-1). detected in paraffin-embedded section of human breast cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 5. IHC analysis using anti- SNRPD2 antibody (A12026-1). detected in paraffin-embedded section of human colon adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 6. IHC analysis using anti- SNRPD2 antibody (A12026-1). detected in paraffin-embedded section of human endometrioid adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 7. IHC analysis using anti- SNRPD2 antibody (A12026-1). detected in paraffin-embedded section of human glioblastoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 8. IHC analysis using anti- SNRPD2 antibody (A12026-1). detected in paraffin-embedded section of human liver cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 9. IHC analysis using anti- SNRPD2 antibody (A12026-1). detected in paraffin-embedded section of human prostate adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 10. IHC analysis using anti- SNRPD2 antibody (A12026-1). detected in paraffin-embedded section of human right renal oncocytoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 11. IHC analysis using anti- SNRPD2 antibody (A12026-1). detected in paraffin-embedded section of human thyroid papillary carcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 12. IHC analysis using anti- SNRPD2 antibody (A12026-1). detected in paraffin-embedded section of human urothelial carcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 13. IF analysis using anti- SNRPD2 antibody (A12026-1). detected in paraffin-embedded section of human squamous carcinoma tissue. The tissue section were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) and counterstained with DAPI (blue).|Figure 14. IF analysis using anti- SNRPD2 antibody (A12026-1). detected in paraffin-embedded section of human breast cancer tissue. The tissue section were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) and counterstained with DAPI (blue).|Figure 15. ICC analysis using anti- SNRPD2 antibody (A12026-1) and anti-Tubulin beta antibody (M05613-4). were detected in immersion fixed SiHa cell line. Cells were stained using the Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) and Dylight488-conjugated Anti- mouse IgG Secondary Antibody (green)(Catalog#BA1126).|Figure 16. Flow cytometry analysis of Jurkat cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-SNRPD2 Antibody(A12026-1-100ul)
￥1960
