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- BOSTER
- A09010-2-100ul
- 中国
- 2025年07月12日
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- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A09010-2 |
|---|---|
| 产品名称 | Anti-SNX18 Antibody |
| 基因名 | SNX18 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 69KD |
| 免疫原 | E.coli-derived human SNX18 recombinant protein (Position: R22-F628). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Sorting nexin-18 is a protein that in humans is encoded by the SNX18 gene. This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein does not contain a coiled coil region, like some family members, but contains a SH3 domain. Multiple transcript variants encoding different isoforms have been found for this gene. |
| 研究类别 | 1. Ha?berg, K., Lundmark, R., & Carlsson, S. R. (2008). SNX18 is an SNX9 paralog that acts as a membrane tubulator in AP-1-positive endosomal trafficking. Journal of cell science, 121(9), 1495-1505.2. Okano, M., Matsuo, H., Nishimura, Y., Hozumi, K., Yoshioka, S., Tonoki, A., & Itoh, M. (2016). Mib1 modulates dynamin 2 recruitment via Snx18 to promote Dll1 endocytosis for efficient Notch signaling. Genes to Cells, 21(5), 425-441.3. Willenborg, C., Jing, J., Wu, C., Matern, H., Schaack, J., Burden, J., & Prekeris, R. (2011). Interaction between FIP5 and SNX18 regulates epithelial lumen formation. Journal of Cell Biology, 195(1), 71-86. |
| Uniprot ID | SNX18: Q96RF0 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| ELISA: | 1:100-1000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- SNX18 antibody (A09010-2). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: rat lung tissue lysates,
Lane 4: rat kidney tissue lysates.
Use rabbit anti- SNX18 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for SNX18 at approximately 69KD. The expected band size for SNX18 is at 69KD.[/list_product_images]
Lane 1: human HL-60 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: rat lung tissue lysates,
Lane 4: rat kidney tissue lysates.
Use rabbit anti- SNX18 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for SNX18 at approximately 69KD. The expected band size for SNX18 is at 69KD.[/list_product_images]
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Anti-SNX18 Antibody(A09010-2-100ul)
￥1960
