- ￥1960
- BOSTER
- M03409-100ul
- 中国
- 2025年07月10日
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- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | M03409 |
|---|---|
| 产品名称 | Anti-SERPINB5 Antibody (monoclonal, 7G4E1) |
| 基因名 | SERPINB5 |
| 抗体来源 | Mouse |
| 克隆 | Monoclonal(Clone:7G4E1) |
| 抗体亚型 | Mouse IgG2a |
| 分子量 | 42KD |
| 免疫原 | E.coli-derived human MASPIN recombinant protein (Position: M1-A350). Human MASPIN shares 88% and 89% amino acid (aa) sequence identity with mouse and rat MASPIN, respectively. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | protein G purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | SERPINB5 is also known as PI5 or maspin. Maspin (mammary serine protease inhibitor) is a protein that in humans is encoded by the SERPINB5 gene. Maspin is expressed in the skin, prostate, testis, intestine, tongue, lung, and the thymus. Maspin is a member of the serpin superfamily of serine protease inhibitors.[1] The primary function of most members of this family is to regulate the breakdown of proteins by inhibiting the catalytic activity of proteinases. Through this mechanism of action, serpins regulate a number of cellular processes includingphagocytosis, coagulation, and fibrinolysis. |
| 研究类别 | 1. Blandamura S, et al. Maspin expression, subcellular localization and clinicopathological correlation in endometrial hyperplasia and endometrial adenocarcinoma. Histol Histopathol, 2014 Jun. 2. Taskiran C, et al. Maspin expression in endometrial hyperplasia and carcinoma, and its relation with angiogenesis. Eur J Gynaecol Oncol, 2014.3. Triulzi T, et al. Maspin influences response to doxorubicin by changing the tumor microenvironment organization. Int J Cancer, 2014 Jun 15. |
| Uniprot ID | SERPINB5: P36952 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P). |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry(Paraffin-embedded Section): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- SERPINB5 Antibody (M03409). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: HACAT whole cell lysates,
Lane 3: CACO-2 whole cell lysates.
Use mouse anti- SERPINB5 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for SERPINB5 at approximately 42KD. The expected band size for SERPINB5 is at 42KD.|Figure 2. IHC analysis using anti- SERPINB5 Antibody (M03409). detected in paraffin-embedded section of human tonsil tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB as the chromogen.|Figure 3. IHC analysis using anti- SERPINB5 Antibody (M03409). detected in paraffin-embedded section of human Laryngeal squamous cell carcinomas tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB as the chromogen.|Figure 4. Flow cytometry analysis of SiHa cell (1x106) DyLight 488 conjugated goat anti- mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: HELA whole cell lysates,
Lane 2: HACAT whole cell lysates,
Lane 3: CACO-2 whole cell lysates.
Use mouse anti- SERPINB5 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for SERPINB5 at approximately 42KD. The expected band size for SERPINB5 is at 42KD.|Figure 2. IHC analysis using anti- SERPINB5 Antibody (M03409). detected in paraffin-embedded section of human tonsil tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB as the chromogen.|Figure 3. IHC analysis using anti- SERPINB5 Antibody (M03409). detected in paraffin-embedded section of human Laryngeal squamous cell carcinomas tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB as the chromogen.|Figure 4. Flow cytometry analysis of SiHa cell (1x106) DyLight 488 conjugated goat anti- mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-SERPINB5 Antibody (monoclonal, 7G4E1)(M03409-100ul)
￥1960
