- ￥1960
- BOSTER
- M08821-2-100ul
- 中国
- 2025年07月15日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | M08821-2 |
|---|---|
| 产品名称 | Anti-H1F0 Antibody (monoclonal, 5I3E6) |
| 基因名 | H1F0 |
| 抗体来源 | Mouse |
| 克隆 | Monoclonal(Clone:5I3E6) |
| 抗体亚型 | Mouse IgG2b |
| 分子量 | 24KD |
| 免疫原 | E.coli-derived human Histone H1.0/H1F0 recombinant protein (Position: K20-K159). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | protein G purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | H1 histone family, member 0?is a member of the?histone?family of nuclear?proteins?which are a component of?chromatin. In humans, this protein is encoded by the?H1F0?gene. It is mapped to 22q13.1. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family. |
| 研究类别 | 1. Doenecke D, T?njes R (February 1986). "Differential distribution of lysine and arginine residues in the closely related histones H1 and H5. Analysis of a human H1 gene". Journal of Molecular Biology. 187 (3): 461–4. 2. Albig W, Drabent B, Kunz J, Kalff-Suske M, Grzeschik KH, Doenecke D (June 1993). "All known human H1 histone genes except the H1(0) gene are clustered on chromosome 6". Genomics. 16 (3): 649–54. |
| Uniprot ID | H1F0: P07305 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P). |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry(Paraffin-embedded Section): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of anti- H1-0 Antibody (M08821-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: U2OS whole cell lysates,
Lane 2: PC-3 whole cell lysates,
Lane 3: HEPG2 whole cell lysates.
Use mouse anti- H1-0 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for H1-0 at approximately 24KD. The expected band size for H1-0 is at 21KD.|Figure 2. IHC analysis using anti- H1-0 Antibody (M08821-2). detected in paraffin-embedded section of mouse colon tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB as the chromogen.|Figure 3. IHC analysis using anti-H1-0 Antibody (M08821-2). detected in paraffin-embedded section of human placenta tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB as the chromogen.|Figure 4. IHC analysis using anti-H1-0 Antibody (M08821-2). detected in paraffin-embedded section of human This is a differentiated adenocarcinoma of the rectum tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB as the chromogen.|Figure 5. IHC analysis using anti-H1-0 Antibody (M08821-2). detected in paraffin-embedded section of human Hodgkin's lymphoma tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB as the chromogen.|Figure 6. IHC analysis using anti-H1-0 Antibody (M08821-2). detected in paraffin-embedded section of human renal cell carcinoma tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB as the chromogen.|Figure 7. IHC analysis using anti-H1-0 Antibody (M08821-2). detected in paraffin-embedded section of human tonsil tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB as the chromogen.|Figure 8. IHC analysis using anti-H1-0 Antibody (M08821-2). detected in paraffin-embedded section of human gastric carcinoma tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB as the chromogen.|Figure 9. IHC analysis using anti-H1-0 Antibody (M08821-2). detected in paraffin-embedded section of human liver cancer tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB as the chromogen.|Figure 10. Flow cytometry analysis of SiHa cell (1x106) DyLight 488 conjugated goat anti- mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: U2OS whole cell lysates,
Lane 2: PC-3 whole cell lysates,
Lane 3: HEPG2 whole cell lysates.
Use mouse anti- H1-0 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for H1-0 at approximately 24KD. The expected band size for H1-0 is at 21KD.|Figure 2. IHC analysis using anti- H1-0 Antibody (M08821-2). detected in paraffin-embedded section of mouse colon tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB as the chromogen.|Figure 3. IHC analysis using anti-H1-0 Antibody (M08821-2). detected in paraffin-embedded section of human placenta tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB as the chromogen.|Figure 4. IHC analysis using anti-H1-0 Antibody (M08821-2). detected in paraffin-embedded section of human This is a differentiated adenocarcinoma of the rectum tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB as the chromogen.|Figure 5. IHC analysis using anti-H1-0 Antibody (M08821-2). detected in paraffin-embedded section of human Hodgkin's lymphoma tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB as the chromogen.|Figure 6. IHC analysis using anti-H1-0 Antibody (M08821-2). detected in paraffin-embedded section of human renal cell carcinoma tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB as the chromogen.|Figure 7. IHC analysis using anti-H1-0 Antibody (M08821-2). detected in paraffin-embedded section of human tonsil tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB as the chromogen.|Figure 8. IHC analysis using anti-H1-0 Antibody (M08821-2). detected in paraffin-embedded section of human gastric carcinoma tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB as the chromogen.|Figure 9. IHC analysis using anti-H1-0 Antibody (M08821-2). detected in paraffin-embedded section of human liver cancer tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB as the chromogen.|Figure 10. Flow cytometry analysis of SiHa cell (1x106) DyLight 488 conjugated goat anti- mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-H1-0 Antibody (monoclonal, 5I3E6)(M08821-2-100ul)
￥1960
