- ￥1960
- BOSTER
- A07933-1-100ul
- 中国
- 2025年07月12日
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- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A07933-1 |
|---|---|
| 产品名称 | Anti-WNT8B Antibody |
| 基因名 | WNT8B |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 40-50KD |
| 免疫原 | E.coli-derived human WNT8B recombinant protein (Position: A206-R340). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Protein Wnt-8b is a protein that in humans is encoded by the WNT8B gene. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 95%, 86% and 71% amino acid identity to the mouse, zebrafish and Xenopus Wnt8B proteins, respectively. The expression patterns of the human and mouse genes appear identical and are restricted to the developing brain. The chromosomal location of this gene to 10q24 suggests it as a candidate gene for partial epilepsy. |
| 研究类别 | 1. Houart, C., Caneparo, L., Heisenberg, C.-P., Barth, K. A., Take-Uchi, M., Wilson, S. W. Establishment of the telencephalon during gastrulation by local antagonism of Wnt signaling. Neuron 35: 255-265, 2002. 2. Lako, M., Lindsay, S., Bullen, P., Wilson, D. I., Robson, S. C., Strachan, T. A novel mammalian Wnt gene, WNT8B, shows brain-restricted expression in early development, with sharply delimited expression boundaries in the developing forebrain. Hum. Molec. Genet. 7: 813-822, 1998. 3. Lako, M., Strachan, T., Curtis, A. R. J., Lindsay, S. Isolation and characterization of WNT8B, a novel human Wnt gene that maps to 10q24. Genomics 35: 386-388, 1996. |
| Uniprot ID | WNT8B: Q93098 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| (ELISA)： | 1:100-1000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- WNT8B Antibody (A07933-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: RT4 whole cell lysates.
Use rabbit anti- WNT8B 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for WNT8B at approximately 40-50KD. The expected band size for WNT8B is at 39KD.|Figure 2. ICC analysis using anti- WNT8B Antibody (A07933-1). was detected in immersion fixed MCF-7 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue). [/list_product_images]
Lane 1: RT4 whole cell lysates.
Use rabbit anti- WNT8B 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for WNT8B at approximately 40-50KD. The expected band size for WNT8B is at 39KD.|Figure 2. ICC analysis using anti- WNT8B Antibody (A07933-1). was detected in immersion fixed MCF-7 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue). [/list_product_images]
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Anti-WNT8B Antibody(A07933-1-100ul)
￥1960
