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广州威佳科技有限公司
100ul
货号 | A03512-3 |
---|---|
产品名称 | Anti-GYS1 Antibody |
基因名 | GYS1 |
抗体来源 | Rabbit |
克隆 | Polyclonal |
抗体亚型 | Rabbit IgG |
分子量 | 84-90KD |
免疫原 | E.coli-derived human Glycogen synthase 1/GYS1 recombinant protein (Position: D60-A640). |
内容 | 500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol. |
纯化方式 | Immunogen affinity purified. |
浓度 | 500 ug/ml |
产品形态 | Liquid |
保存条件 | 12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
背景资料 | Glycogen synthase (UDP-glucose-glycogen glucosyltransferase) is a key enzyme in glycogenesis, the conversion of glucose into glycogen. The protein encoded by this gene catalyzes the addition of glucose monomers to the growing glycogen molecule through the formation of alpha-1,4-glycoside linkages. Mutations in this gene are associated with muscle glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
研究类别 | 1. Browner, M. F., Nakano, K., Bang, A. G., Fletterick, R. J. Human muscle glycogen synthase cDNA sequence: a negatively charged protein with an asymmetric charge distribution. Proc. Nat. Acad. Sci. 86: 1443-1447, 1989. 2. Cameron, J. M., Levandovskiy, V., MacKay, N., Utgikar, R., Ackerley, C., Chiasson, D., Halliday, W., Raiman, J., Robinson, B. H. Identification of a novel mutation in GYS1 (muscle-specific glycogen synthase) resulting in sudden cardiac that, that is diagnosable from skin fibroblasts. Molec. Genet. Metab. 98: 378-382, 2009.3. Groop, L. C., Kankuri, M., Schalin-Jantti, C., Ekstrand, A., Nikula-Ijas, P., Widen, E., Kuismanen, E., Eriksson, J., Franssila-Kallunki, A., Saloranta, C., Koskimies, S. Association between polymorphism of the glycogen synthase gene and non-insulin-dependent diabetes mellitus. New Eng. J. Med. 328: 10-14, 1993. Note: Erratum: New Eng. J. Med. 328: 1136 only, 1993. |
Uniprot ID | GYS1: P13807 |
推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC. |
为了提供优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
---|---|
Western blot(WB): | 1:500-2000 |
Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
Flow cytometry (FCM): | 1-3μg/1x106 cells |
(ELISA): | 1:100-1000 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
*稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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